Samples of Restriction Enzymes
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
| BamHI | Bacillus amyloliquefaciens |
5'GGATCC 3'CCTAGG |
5'---G GATCC---3' 3'---CCTAG G---5' |
| HindIII | Haemophilus influenzae |
5'AAGCTT 3'TTCGAA |
5'---A AGCTT---3' 3'---TTCGA A---5' |
| TaqI | Thermus aquaticus |
5'TCGA 3'AGCT |
5'---T CGA---3' 3'---AGC T---5' |
| AluI* | Arthrobacter luteus |
5'AGCT 3'TCGA |
5'---AG CT---3' 3'---TC GA---5' |
| * = blunt ends | |||
Chart modified from Wikipedia