Immune-Endocrine control of Leydig cell function

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IN VIVO METHODS

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Steroidogenic Acute Regulatory Protein: StAR

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LPS vs. StAR mRNA expression

LPS vs. StAR Synthesis: 2 hr

LPS vs. StAR synthesis: 2 hr

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What mediates the acute LPS inhibition?

Carbonyl cyanide m-chlorophenylhydrazone (cccp)

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CCCP vs. Progesterone in MA10s

Effect of CCCP on  StAR protein

Effect of CCCP on StAR synthesis

Effect of CCCP on protein synthesis

Effect of CCCP on StAR synthesis

Effect of CCCP on StAR mRNA

Tetramethylrhodamine
Ethyl Ester (TMRE)

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H₂O₂ vs. Progesterone in MA10s

H₂0₂ vs. StAR and 3b-HSD protein in MA10s

Effect of H₂O₂ on StAR mRNA

TMRE staining of MA-10 cells exposed to H2O2

Summary: StAR and 3b-HSD studies in MA10s

Hypothesis-one

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Summary: in vivo studies

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