BIOCHEMISTRY OF MUSCLE CONTRACTION
This home page contains selected graduate course lectures, PHYB-BCHE 516, 1975-1997, by Michael and Kate Bárány, University of Illinois at Chicago
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BIOCHEMISTRY OF MUSCLE CONTRACTION This home page contains selected graduate course lectures, PHYB-BCHE 516, 1975-1997, by Michael and Kate Bárány, University of Illinois at Chicago Updated: March 2002 |
Myosin
Structure
Size and shape of the myosin molecule
Myosin light chains
Review
Function
Myosin-actin binding
ATPase activity of myosin
Separate actin-binding and ATPase sites of myosin
Intermediates of the ATP hydrolysis
ATPase activity of myosin and speed of muscle shortening
Myosin heavy chains
Three dimensional structure of subfragment 1
Structure-function relationship in myosin
Assembly
Myosin filamentMuscle fibers, myofibrils
Localization of myosin in the structure of muscle
References
Actin
The two forms of actinActin-myosin binding
Three dimensional structure of actin
The intersubunit contacts in the F-actin filaments
Localization of actin in the structure of muscle
Structure of the thin filament
References
Actin-Myosin Interaction
Structural modelContact sites
Lever arm model
X-ray diffraction and electron microscopy of muscle
In vitro motility assay
References
Tropomyosin
Tropomyosin isoforms
Structure of tropomyosin
Binding properties
Troponin
Troponin CTroponin I
Troponin T
References
The Role of Ca2+ in Regulation of Skeletal Muscle Contraction
Historical experimentsThe experiments of Huxley and Taylor
Sarcoplasmic Reticulum
Signal transduction between T-tubule and SR-junctionExcitation-Contraction Coupling
Sequence of eventsReferences
The sliding filament theoryLength-tension relationship
Crossbridge cycle and its relation to actomyosin ATPase
Summary of Events in Skeletal Muscle Contraction
Excitation
Contraction
Relaxation
References
Historical Development of Muscle Energetics
The lactic acid theory and its disproofThe Lohmann reaction and its inhibition
ATP, Phosphocreatine and Glycogen Provide Energy for Muscle Contraction
Heat Production during Muscle Contraction
The relationship between energy output and chemical breakdownEnergy Cost Assessment in Humans
Direct calorimetryIndirect calorimetry
Relationship between work-output and O2 consumption
Oxygen debt
Adaptation to exercise
Fatigue
References
Basic physiology
Ultrastructure
Myosin
Actin
Troponin C
Troponin I
Troponin T
Tropomyosin
Movement of the regulatory proteins in systole versus diastole
Methods
Displacement of endogenous TN in skinned fibers with TN mutants
Purification of cardiac myofibrils
Gel electrophoresis of the regulatory proteins
Literature
Sarcoplasmic Reticulum
Ca2+ induced Ca2+ release
Calsequestrin
Phospholamban
Factors controlling the Ca2+ release from SR
Sarcolemma
Major events in cardiac E-C coupling
Suggested readings
References
StructureInnervation and stimulation
Myofibril proteins
Myosin light chain kinase and myosin light chain phosphataseMyosin light chain phosphorylation in intact smooth muscle
Isoforms of the 20-kDa myosin light chain
Phosphorylation site
Two-dimensional tryptic peptide mapping
The role of Ca2+ in light chain phosphorylation
Stretch-induced light chain phosphorylation
Phosphorylation of Heat Shock Proteins
Inositol 1,4,5-trisphosphateG-proteins
Phosphoinoside-specific phospholipase C
Characteristics of the exchange of the actin-bound nucleotide in smooth muscle
References
Historical Development of Cell Motility
Profilin
Gelsolin
Structure and functionADF/cofilin
Arp2/3 complex and WASp/Scar proteins
Dendritic nucleation model
Actin in the cytoskeleton
Kinesin and dyneinReferences
For more information please contact:
Michael Bárány
Department of Biochemistry and Molecular Biology (M/C 536)
University of Illinois at Chicago
Chicago, IL 60612-7334