ADDRESS
Section of Digestive Diseases and Nutrition
University of Illinois at Chicago
840 South Wood Street (M/C 716)
Chicago, IL 60612
Telephone: 312-996-6651
Fax: 312-996-5103
E-mail
RESEARCH INTERESTS
Na+/H+ exchangers catalyze the electroneutral exchange of an extracellular Na + for an intracellular H + . These proteins are involved in a variety of physiological processes such as Na + , bicarbonate and water absorption and regulation of pH i , cell proliferation and differentiation. Of nine isoforms identified to date (NHE1-NHE9), NHE1, NHE2, and NHE3 have been described in the human intestinal tract. Our lab is interested in understanding the mechanisms involved in the transcriptional regulation of the human Na + /H + exchangers, NHE2 and NHE3. Using a variety of molecular and biochemical approaches we have cloned the promoter region for both NHE2 and NHE3 isoforms. Since gene regulation can be controlled by transcription factors binding to the specific cis-elements in the promoters, we have identified a number of zinc-finger DNA binding transcription factors that recognize the same DNA motif in NHE2 and NHE3 promoters by mutational analyses and in vitro DNA-protein binding experiments. We are using Chromatin Immunoprecipitation experiments to identify which transcription factors bind these promoters in vivo. In addition, we are interested in understanding the regulatory pathways that modulate the DNA-protein and protein-protein interactions. Furthermore, experiments are in progress to determine the effects of the epigenetic modification of the NHE2 and NHE3 promoters on the transcriptional regulation of these genes and its relationship to the cell and region specific NHE expression.
EDUCATION AND TRAINING
PhD in Biological Sciences - University of Illinois at Chicago, Chicago, Illinois
Postdoctoral Fellow - Department of Biological Sciences, University of South Carolina
PUBLICATIONS
• R.K. Gill, S. Saksena, S. Tyagi, W.A. Alrefai, J. Malakooti, J. Turner, K. Ramaswamy, P.K. Dudeja: Serotonin inhibits Na+/H+ exchange activity via 5-HT4 receptor subtypes and activation of PKC a in Caco-2 cells. Gastroenterology (In Press)
• G. Hecht, K. Hodges, RK. Gill, F Kear, S Tyagi , J Malakooti, K Ramaswamy and PK Dudeja. 2004. Differential Regulation of Na+/H+ Exchange Isoform Activities by enteropathogenic E. coli in Human Intestinal Epithelial Cells. Am. J. Physiol 287:G370-378
• Malakooti, J., V.C. Memark, P. K. Dudeja, and K. Ramaswamy. 2002. Molecular cloning and characterization of the human Na + / H + exchanger NHE-3 promoter: transcription factor AP-2 is involved in NHE-3 regulation. Am. J. Physiol., 282:G491-500
• R.K. Gill, S. Saksena, S. Irfan, S. Tyagi, W.A. Alrefai, J. Malakooti, K. Ramaswamy and P.K. Dudeja. 2002. Regulation of NHE3 by by nitric oxide in Caco-2 cells. Am. J. Physiol. 283:G747-G756
• Malakooti, J., R. Dahdal, L. Schmidt, P. K. Dudeja, T. J. Layden, and K. Ramaswamy. 2001. The human Na + /H + exchanger isoform 2 (NHE-2) gene: genomic organization, chromosomal localization, and Promoter characterization. Am. J. Physiol., 280:G763-2773
• Malakooti, J., R. Dahdal, L. Schmidt, P. K. Dudeja, T. J. Layden, and K. Ramaswamy. 1999. Molecular cloning, tissue distribution and expression of the human Na + /H + exchanger, NHE-2. Am. J. Physiol. 277: G383-390
• Mangan, EK., J. Malakooti, A Caballero, B. Ely, and JW. Gober. 1999. FlbT couples flagellum assembly to gene expression in Caulobacter crescentus. J. Bacteriol., 181:6160-70
• Malakooti, J., S.P. Wang, and B. Ely. 1995. A consensus promoter sequence for Caulobacter RNA polymerase. J. Bacteriol. 177: 6854-6860
• Malakooti, J., S.P. Wang, and B. Ely. 1995. A consensus promoter sequence for Caulobacter crescentus genes involved in biosynthetic and housekeeping functions. 1995. J. Bacteriol 177:4372-4386
• Tarleton, J., J. Malakooti, and B. Ely. 1994. Regulation of Caulobacter crescentus ilvB gene expression. J. Bacteriol. 176: 3765-3774
• Malakooti, J., and B. Ely. 1994. Identification and characterization of the ilvR gene, a LysR-type regulator of the Caulobacter crescentus. J. Bacteriol. 176: 1275-1281
• Malakooti, J., B. Ely, and P. Matsumura. 1994. Molecular characterization, nucleotide sequence and expression of four flagellar genes, fliO, fliP, fliQ, and fliR of Escherichia coli: FliP is a membrane protein with N-terminal signal sequence. J. Bacteriol. 176: 189-197
• Malakooti, J., Y. Komeda, and P. Matsumura. 1989. DNA sequence analysis, gene product identification and localization of flagellar motor components of Escherichia coli. J. Bacteriol. 171:2728-2734
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