 K. (Ram) Ramaswamy, Ph.D.
Professor of Physiology in Medicine
Address
Section of Digestive Diseases and Nutrition
University of Illinois at Chicago
840 S. Wood Street (M/C 716)
Room 718E
Chicago, Illinois 60612
Telephone: 312-996-0899
Fax: 312-996-5103
Education and Training
College: Madras University, Madras, India (B.S., 1960)
Graduate School: Madras University, Madras, India (M.S., 1962, Ph.D., 1968)
Postdoc: Rutgers Medical School, NJ (1969-1971)
Basic Research Interests
My research efforts are focused on elucidating the mechanisms of Na+ and Cl- transport in the human intestine utilizing biochemical, cell and molecular biology techniques. Specifically the roles of Na+/H+ (NHE) exchanger isoforms in Na+ transport and Cl-/HCO3- exchanger (AE) isoforms in Cl- and other anion transport in the human small intestine and colon are being addressed. Current studies include studies of transcriptional and post-translational regulation of the human NHE-2 and NHE-3 isoforms.
Since short-chain fatty acids such as butyrate and propionate are the preferred substrates for metabolism for colonocytes, ongoing studies include studies to clone the transporter for short-chain fatty acids and characterize the mechanisms for transport in the human colon.
Representative Publications
• J.M. Harig, E.K. Ng, P.K. Dudeja, T.A. Brasitus and K. Ramaswamy: Transport of n-butyrate into human colonic luminal membrane vesicles. Am. J. Physiol. 271 (Gastroint. Liver Physiol. 34): G415-422, 1996.
• P.K. Dudeja, D.D. Rao, I. Syed, V. Joshi, R.Y. Dahdal, C. Gardner, M.C. Risk, L. Schmidt, D. Bavishi, K.E. Kim, J.M. Harig, T.J. Layden and K. Ramaswamy : Intestinal distribution of Na + /H + exchanger isoforms NHE-1, NHE-2 and NHE-3 mRNA. Amer. J. Physiol. 271 (Gastroint. Liver Physiol. 34): G483-493, 1996.
• J. Malakooti, R.Y. Dahdal, L. Schmidt, P.K. Dudeja, T.J. Layden and K. Ramaswamy : Molecular cloning, tissue distribution and expression of human Na+/H+ exchanger, NHE-2 Am. J. Physiol 277(Gastroint. Liver Physiol. 40): G383-90, 1999.
• C. Hadjiagapiou, L. Schmidt, P.K. Dudeja, T.J. Layden and K. Ramaswamy: Mechanism(s) of butyrate transport in Caco-2 cells: Role of monocarboxylate transporter 1. Am. J. Physiol (Gastroint. Liver Physiol.) 279(4): G775-G780, 2000.
• J. Malakooti, R.Y. Dahdal, P.K. Dudeja, T.J. Layden and K. Ramaswamy: The human Na + /H + exchanger isoform NHE-2 gene: genomic organization and promoter characterization. Am. J. Physiol . 280: G763-G773, 2001.
• W.A. Alrefai, B. Scaglione-Sewell, S. Tyagi, L. Wartman, T.A. Brasitus, K. Ramaswamy and P.K. Dudeja: Differential Regulation of the Expression of Na + /H + exchanger isoform NHE3 by PKC a in CaCo2 cells. Am. J. Physiol . 281:C1551-8, 2001..
• Malakooti, V.C. Memark, P.K. Dudeja, and K. Ramaswamy. Molecular cloning and functional analysis of the human Na + /H + exchanger NHE3 promoter. Am. J. Physiol, (Gastroint. & Liver Physiol.), 282:G491-500, 2002.
• S. Saksena, R.K. Gill, I.A. Syed, S. Tyagi, W.A. Alrefai, K. Ramaswamy and P.K. Dudeja. Modulation of Cl - / OH - exchange activity in Caco-2 cells by nitric oxide. Am. J. Physiol. 283:G626-33, 2002.
• R.K. Gill, S. Saksena, I.A. Syed, S. Tyagi, W.A. Alrefai, J. Malakooti, K. Ramaswamy and P.K. Dudeja. Regulation of NHE3 by nitric oxide in Caco-2 cells. Am. J. Physiol. 283: G747-56, 2002.
• S. Saksena, R.K. Gill, I.A. Syed, S. Tyagi, W.A. Alrefai, K. Ramaswamy and P.K. Dudeja. Inhibition of the apical Cl - / OH - exchange activity in Caco-2 cells by phorbol esters is mediated by protein kinase C e. Am. J. Physiol. (Cell Physiol) 283:C1492-500, 2002.
• W.A. Alrefai, S. Tyagi, R. K. Gill, S. Saksena, C. Hadjiagapiou, F. Mansour, K. Ramaswamy, P. K. Dudeja: Regulation of butyrate uptake in Caco-2 Cells by Phorbol 12-Myristate 13-Acetate, PMA. Am. J. Physiol . 286:G197-203, 2004.
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