Core Research Facilities
Cell Biology Module
Dr. Beatrice Yue, Module Director |
312-996-6125 |
Martin Tibudan, Research Specialist, Tissue Culture |
312-996-0062 |
Cynthia Haynes, Research Specialist, Morphology |
Contact Dr. Yue directly for Morphology service. |
The Cell Biology Module offers dedicated equipment and expertise in establishing, maintaining and propagating normal, transformed, and transfected cell lines and organ cultures for use in ophthalmic research. Beatrice Yue, Ph.D. directs the module. Dr. Yue, a cell biologist with over 20 years of experience in tissue culture and morphologic experiment, is well-regarded for her work on the trabecular meshwork.
The module’s tissue culture services include cell counting, cell freezing, and cell plating for tissue culture experiments, cell transfection, and microinjection. The module also provides the required facilities and expertise to perform morphological studies of tissue specimens and cultured cells.
The Cell Biology Module occupies approximately 580 sq. ft. of dedicated space on the second floor of the LIERI Building , with one tissue culture room , one histology/tissue preparation laboratory, and one autoclave/purified water room. Major equipment includes:
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Two Sanyo vertical tissue culture CO 2 incubators
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Baker Steri-guard laminar tissue culture hood
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Eppendorf Transjector 5246 microinjection unit equipped with Nikon Diaphot TMD phase contrast and fluorescence microscope with Olympus DP10 digital camera
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Beckman-Coulter Z1 dual cell counter
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Zeiss Axiovert 25, Leitz Wetzlar Diavert and Olympus BH-2 phase contrast/fluorescence microscopes
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Jouan BR41 centrifuge, Avanti J25 centrifuge, and Beckman L80 ultracentrifuge
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Barnstead-Thermolyne ROPure Infinity Premier Moel reverse osmosis system
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Continental and Tuttnauer 3870 EA autoclaves
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Lipshaw Technicon tissue processor and Shandon Lipshaw embedding center
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Reichert-Jung ultramicrotome
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IEC cryostat
