Core Research Facilities
Cellular and Molecular Biology Module
Dr. Beatrice Yue, Module Director |
312-996-6125 |
Hongyu Ying, Research Specialist |
312-413-4194 |
The Cellular and Molecular Biology Module offers dedicated equipment and expertise for tissue culturing, histologic analyses and molecular biology experiments. Beatrice Yue, PhD, directs the module. Dr. Yue, a cell biologist with over 25 years of experience in cell biology and molecular biology studies, is well-regarded for her work on the cornea and the trabecular meshwork.
The module’s tissue culture services include cell counting, cell freezing, and cell plating for tissue culture experiments, cell transfection, and microinjection. The module also provides the required facilities and expertise to perform morphological studies of tissue specimens and cultured cells. In addition, the module offers consultation and services related to molecular biological techniques, such as DNA sequencing, RNA preparation, real-time PCR, and proteomics with SELDI technology
The Cellular and Molecular Biology Module is housed on the second floor of the LIERI Building, with one tissue culture room, one histology/tissue preparation laboratory, one molecular biology room, and one autoclave/purified water room. Major equipment includes:
- Barnstead-Thermolyne ROPure reverse osmosis and NANOpure ultrapure water systems.
- Consolidated MK II and Tuttnauer 3870 EA autoclaves.
- Beckman-Coulter Z1 dual cell counter, Eppendorf Transjector 5246 and Injectman microinjection unit.
- BTX electroporation system and Amaxa Nucleofector II.
- Leitz CI 249, lEC IM-179, and American Optical 820 microtomes, and Reichert-Jung ultramicrotome.
- Beckman Avanti J20XP and J25 high speed centrifuges, Beckman L8-70M ultracentrifuge and rotors.
- Cepheid Smart Cycler for real time PCR.
- Kodak Digital Science 440CF Image station and Packard Cyclone phosphorimager.
- Bio-Rad Gel Doc 2000 photodocumentation system and Tecan GENios pro microplate reader.
- Beckman LS3801 scintillation counter, Ciphergen ProteinChip system.
- Nanodrop spectrophotometer, Perkin Elmer LS 50B fluorometer, and OPTIMAX X-ray film developer.