Qingbo Li

Assistant Professor

Ph.D. Iowa State University, 1995

Room: 3220 MBRB, Tel: 312-413-9301

Email: qkli@uic.edu

We are interested in the role and the pathway of anaerobic respiration by M. tuberculosis for intracellular growth and persistence. Even though M. tuberculosis is classified as an obligate aerobe, its successful survival in macrophages and granuloma suggests that it may carry out anaerobic respiration. Indeed, the genome of M. tuberculosis contains two anaerobic nitrate reductase gene clusters, in addition to a large number of genes for fatty acid metabolism and lipid biosynthesis. The ability for M. tuberculosis to carry out anaerobic respiration is probably a critical molecular basis for long term persistence. Currently there is a paucity of information about the protein expression of M. tuberculosis in infected macrophages. Recent advances of proteomics, particularly in quantitation capability, have provided great promise of benefits to biology community. Given that cells are dynamic with metabolisms that can react to an ever-changing environment, our true understanding of a proteome will require information of both dynamic and homeostatic mechanisms, plus an understanding of the governing intra- and extra-cellular components, including host immune response. Using proteomics approach, we expect to define the proteins critical for M. tuberculosis persistence in infected macrophages and granuloma tissues, and the implication of these proteins for drug discovery.