Imaging
and Microscopy Core
The puropose of the Core is to strengthen and support the research of the U54 and other investigators by providing state-of-the-art histology services
The Core is located in the College of Medicine Research Building in new laboratory space with centralized equipment and services in one location
Services provided by Core:
- Training and consultation for Center personnel
- Consultation of the various fixation methods for tissues is available to those who require assistance
- Dehydration of fixed tissues
- Tissues are embedded in paraffin and processed for sectioning
- Tissue sectioning is done either by our Research Specialist or are made available to those who wish to use it themselves
- Cryosectioning is done with our state-or-art cryostat
- Routine Hematoxylin and Eosin staining of tissue sections
- Antibody testing for determination of optimal conditions
- Immunofluorescence of cultured cells
- Tissue preparation for electron microscopy
- Slide analysis
- Antibody purification for use in immunohistochemistry
- TUNEL staining in fixed sections for apoptosis studies
- Dual color TUNEL and PCNA or Ki-67 staining for apoptosis/proliferation index
- In situ hybridization for localizing mRNA in tissue sections (fluorescent and colorometric visualization)
- Prepare tissue samples for confocal microscopy and/or electron microscopy at the Research Resources Center (RRC)
Image analysis
The Core provides services to assist investigators with image acquisition and documentation of results using our image analysis workstation. Using the Nikon Eclipse E400 series microscope, single images are captured, processed and printed with a fully functional computer workstation. The Image-Pro Plus software and NIH Image J are used for quantitative morphometric analyses.
Histology training center
The Core trains investigators, pre- and post-doctoral students as well as technicians of U54 investigators in the art and science of immunohistochemistry, in situ hybridization, image processing and analysis.
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