Research interests in our laboratory focus on the role of platelet adhesive functions on hemostasis and thrombosis. Specifically, we are studying the structure-function relationship of integrin alphaIIbß3 and the mechanism by which this transmembrane adhesion receptor is attached to the platelet cytoskeleton.

Techniques Employed: 1) Immunochemistry 2) Molecular and Cellular Biology 3) Protein biochemistry.

Lab Members

• Schober, J.M., Lau, L.F., Ugarova, T.P. and Lam, S.C.-T. Identification of a novel integrin AlphaMß2 binding site in CCN1(CYR61), a matricellular protein expressed in healing wounds and atherosclerotic lesions. Journal of Biological Chemistry, 278(28):25808-25815, 2003.


• Schober, J.M., Chen, N., Grzeszkiewicz, T.M., Jovanovic, I., Emeson, E.E., Ugarova, T.P., Ye, R.D., Lau, L.F. and Lam, S.C.-T. Identification of integrin alphaMß2 as an adhesion receptor on peripheral blood monocytes for Cyr61 (CCN1) and connective tissue growth factor (CCN2): immediate-early gene products expressed in atherosclerotic lesions. Blood, 99(12):4457-4465, 2002.


• Xing, B., Jedsadayanmata, A. and Lam, S.C.-T. Localization of an integrin binding site to the C terminus of talin. Journal of Biological Chemistry, 276(48):44373-44378, 2001.


• Rand, M.L., Packham, M.A., Taylor, D.M., Yeo, E.L., Gemmell, C.H., Patil, S. and Lam, S.C.-T. The fibrinogen chain dodecapeptide inhibits agonist-induced aggregation of rabbit platelets and fibrinogen binding to rabbit glycoprotein IIb-IIIa. Thrombosis and Haemostasis, 82:1680-1686, 1999.


• Patil, S., Jedsadayanmata, A., Wencel-Drake, J.D., Wang, W., Knezevic, I. and Lam, S.C.-T. Identification of a talin- binding site in the integrin ß3 subunit distinct from the NPLY regulatory motif of post-ligand binding functions. Journal of Biological Chemistry, 274(40):28575-28583, 1999.


• Jedsadayanmata, A., Lau, L.F. Chen, C.-C., Kireeva, M.L. and Lam, S.C.-T. Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin AlphaIIbß3. Journal of Biological Chemistry, 274(34):24321-24327, 1999.


• Lau, L.F. and Lam, S.C.-T. The CCN family of angiogeneic regulators: the integrin connection. Experimental Cell Research, 248:44-57, 1999.


• Leisner, T.M., Wencel-Drake, J.D., Wang, W. and Lam, S.C.-T. Bidirectional transmembrane modulation of integrin alphaIIbß3 conformations. Journal of Biological Chemistry, 274(18):12945-12949, 1999.