orbit may be biopsied to diagnose inflammations including infections,
and neoplasms. The age of the patient, the presenting symptoms,
and imaging studies should allow the ophthalmologist to construct
a differential diagnosis. The differential diagnosis, in turn, will
assist the ophthalmologist in handling the biopsy tissue to take
maximum advantage of the partnership with the pathologist in rendering
an accurate and timely diagnosis.
rhabdomyosarcoma from the orbit of a young child.
culture media on hand and be certain that the microbiology laboratory
is aware of the impending arrival of the biopsy sample
should be placed in fixative for permanent sections (please check
with the laboratory for the preferred fixative of choice for suspected
tissue should be sent to the immunopathology laboratory for flow-cytometry
and/or immunohistochemistry. In general, we find that immunohistochemistry
on fresh material is superior to that which can be obtained from
fixed material. Material which is frozen can also be analyzed by
molecular pathology techniques.
alert the laboratory in advance so that the tissue may be processed
in a rapid cycle. Immunohistochemistry may be performed on formalin-fixed
sure to sample viable tissue. If the ophthalmologist samples friable
tissue only, and the tumor is necrotic, it may be difficult to render
may be required to determine the histogenesis. If the patient has
a known primary lesion, and the ophthalmologist suspects a metastasis
to the orbit, please contact the laboratory in advance of the biopsy
for possible special instructions in handling tissue.
the University of Illinois at Chicago (UIC), frozen sections from
orbital tissue are performed in limited circumstances to
the presence of mucormycosis
the margins of resection
if representative tissue has been obtained
in very special circumstances will a diagnosis be rendered on frozen
section material from orbital biopsies before consulting with permanent
sections and marker studies.
orbital lymphomas should be processed specially according to guidelines
for handling lymph nodes.
addition to the ophthalmologist's differential diagnosis, the pathologist
can build a differential diagnosis based upon the age of the patient.
Certain neoplasms tend to occur in children; others in adults. For
example, lymphoma of the orbit seldom occurs in children (outside
of the the context of Burkitt's lymphoma).
you have more questions about orbital biopsy specimens, please contact
one of us:
about another test