These are RGB (TIFF and JPEG) images of 2 cells that were
infected with HIV for about 4 hours. Microtubules are red,
nuclei are blue and HIV particles are green.
HIV 2D -- Single focal plane through the middle
of the cell. Note microtubules on the edges of the cells
are out of focus. Note also that the microtubules are better
defined in the single plane image-the HIV particles can
be seen clearly in association with the microtubules.
JPEG, (747 x 800), 213KB
TIF Format (zip file), 9MB
Time-lapse of a living cell that was injected with fluorescent
tubulin (red), which incorporated into the microtubule structure
of the cell. The nucleus is to the lower left, out of frame.
The cell was infected with HIV virus particles (green) labeled
with green fluorescent protein for a short time (about 30
minutes). Consequently, the particles are still near the
periphery of the cell. Images were taken every minute for
the whole 23-minute movie at 6 frame/second. The cell comes
in an out of focus a little.
Higher Resolution (zip file),
HIV 3D -- 3D, or volume reconstruction of all the
focal planes of the cell projected into a single plane.
Shows the distribution of HIV throughout the whole cell.
JPEG, (747 x 800), 245KB
TIF Format (zip file), 7MB