|
Endogenous Control Primer Sequences:
|
Gene |
Forward Primer Sequence |
Length (bases) |
Tm (degC) |
Reverse Primer Sequence |
Length (bases) |
Tm (degC) |
Expected Size from cDNA (bp) |
Expected Size from Genomic DNA (bp) |
|
GAPDH |
TTGCCATCAATGACCCCTTCA |
21 |
58.4 |
CGCCCCACTTGATTTTGGA |
19 |
56.7 |
173 |
392 |
|
b-actin |
CGTCTTCCCCTCCATCG |
17 |
54.8 |
CTCGTTAATGTCACGCAC |
18 |
51.7 |
94 |
- |
|
b2M |
CCTGAATTGCTATGTGTCTGGG |
22 |
56.9 |
TGATGCTGCTTACATGTCTCGA |
22 |
57.7 |
- |
- |
|
18s rRNA |
GTAACCCGTTGAACCCCATT |
20 |
56.4 |
CCATCCAATCGGTAGTAGCG |
20 |
55.8 |
- |
- |
|
c-myc |
TCAAGAGGTGCCACGTCTCC |
20 |
60.1 |
TCTTGGCAGCAGGATAGTCCTT |
22 |
59.5 |
- |
- |
|
