Where to Start:
Contact information:
Instrumentation: The ABI Prism 7900HT Sequence Detection
System is located in the DNA
Services Facility
A-310 College of Medicine West (CMWT). Currently 96-well and 384-well
micro-plate formats are supported. This system
supports three types of assays: quantitative assays, allelic discrimination
and plus/minus assays. Sign up for the insturment by contacting
the DNA Services Facility at
(312) 355-0679 or email at oligos@mail.rrc.uic.edu
Software availability: Both the Core Genomics and
DNA Sequencing Facilities provide computer work stations supplied
with software packages for data analysis (ABI PRISM SDS2.1) and for
primer and probe design (ABI PRISM Primer Express). When designing
only PCR primer pairs, other general primer design programs could
be used such as Primer Quest available free of charge at: http://biotools.idtdna.com/primerquest/ and Primer Select by DNAstar (Madison, WI). Both of which are available
at Core Genomics Facility computer station. Please refer to the section
Primer and Probe Design for recommended guidelines.
Reading Materials: All background information covering real-time
PCR an end-point assays theory, applications, set up, and troubleshooting
is available at http://www.appliedbiosystems.com.
Hard copies of these materials are also available at both Genomics
and Sequencing Facilities. A short description of the methods,
theoretical introduction and and tips for experimental design and
data analysis are available through the links on the top of this
page.
Services available:
· Introductory training
session
· Individual equipment
use
· Assistance with data analysis
Fee Schedule:
· Introductory training
session: $25 per 30 minutes
· ABI 7900HT instrument
use: $10 per run
· Additional training/assistance:
$25 per hour
