Our Laboratory is located in the Molecular Biology Research Building. The new lab. is well equipped to carry out all aspects of the research conducted in a structural biology laboratory:
Expression of recombinant proteins
conduct analytical expression experiments with our small shaker. Large scale (up
to 12 liters at once!) is done with our brand new (actually, everything is brand
new in this lab.) INFORS shaker. This great shaker does not need clamps to hold
the flasks, and has cooling capabilities. The latter is important in preventing
or reducing the amount of inclusion-body formation.
We have access to various centrifuges, and are especially happy with our Sorval
3B centrifuge that can spin up to 6 liters of culture at a time!
We currently have three purification
systems: for low pressure we use the Bio-Rad Econo System, mostly for affinity
chromatography (e.g. His-tag, or GST-tag). In addition, we have two higher
pressure systems, a Pharmacia FPLC Classic, and a Waters System.
These we use with our Hi-Load gel-filteration
columns and for running ion-exchange chromatorgraphy (i.e. Mono Q).
X-ray data collection
Our in-house X-ray equipment consists of a Rigaku rotating
anode generator and two area detectors. One, an RaxisIV, the
other an RaxisIIc image plate systems. For both we have a cryo-system. The
data collection is controlled from two Pentium PCs.
Structure solution and refinement
Celebrating new and exciting structures