Our Laboratory is located in the Molecular Biology Research Building.

The new lab. is well equipped to carry out all aspects of the research conducted in a structural biology laboratory:

    Expression of recombinant proteins

We conduct analytical expression experiments with our small shaker. Large scale (up to 12 liters at once!) is done with our brand new (actually, everything is brand new in this lab.) INFORS shaker. This great shaker does not need clamps to hold the flasks, and has cooling capabilities. The latter is important in preventing or reducing the amount of inclusion-body formation. We have access to various centrifuges, and are especially happy with our Sorval 3B centrifuge that can spin up to 6 liters of culture at a time!

    Protein purification

We currently have three purification systems: for low pressure we use the Bio-Rad Econo System, mostly for affinity chromatography (e.g. His-tag, or GST-tag). In addition, we have two higher pressure systems, a Pharmacia FPLC Classic, and a Waters System. These we use with our Hi-Load gel-filteration columns and for running ion-exchange chromatorgraphy (i.e. Mono Q).

    X-ray data collection

Our in-house X-ray equipment consists of a Rigaku rotating anode generator and two area detectors. One, an RaxisIV, the other an RaxisIIc image plate systems. For both we have a cryo-system. The data collection is controlled from two Pentium PCs.