Lipton Lab

Jilao Fan

Research Associate Professor
COMRB Room 7080
tel. (312) 355-3597
fax. (312) 355-3581
jlfan@uic.edu

Professional Experience

2005-present Research Associate Professor. Departments of Neurology and Rehabilitation Medicine and Microbiology & Immunology, College of Medicine, University of Illinois at Chicago. Research on autoimmune Multiple Sclerosis (MS) disease: 1) Research on role of the Theiler’s murine encephalomyelitis virus (TMEV) proteins in apoptosis; 2) Search for autoimmune MS susceptibility genes by microarrays analysis; 3) Explore MS susceptibility genes functions using RNA interference (RNAi) techniques for evaluation of potential therapy.

2003-2005 Research Assistant Professor . Brodie Laboratory for Craniofacial Genetics, Department of Oral Biology, College of Dentistry, University of Illinois at Chicago. 1) Investigate structure-function relationship of amelogenin and cp27 proteins. 2) Express recombinant proteins including CP27 protein, amelogenin and its domain peptides, and ameloblastin, by eukaryotic and prokaryotic expression systems, especially using baculovirus expression system. 3) Generate crystallization of amelogenin and cp27 protein for the proteins microstructure research. 4) Research function of CP27 protein in tooth development. 5) Supervise post-doctoral and junior fellows.

2001-2003 Research Assistant Professor. Department of Physiology and Biophysics of Protein Research laboratory, Research Resources Center, University of Illinois at Chicago. 1) Express recombinant nuclear myosin-I by baculovirus expression system. 2) Research on role of nuclear mayosin-I in transcription. 3) Produce, purify, and characterize monoclonal, polyclonal and antipetide antibodies. 4) Purify recombinant proteins by immunoaffinity and HLPC purification methods.

1999-2000 Instructor. Section of Pulmonary and Critical Care, Department of Medicine, The University of Chicago. 1) Researched on soluble HLA peptide inhibitor to treat cockroach sensitive Asthmatics. 2) Studied mechanisms to modulate T cells responses in the setting of pancreatic islet cell allgrafts into juvenile diabetic monkey.

1997-1999 Research Assistant Professor. Department of Microbiology and Immunology, University of Illinois at Chicago. 1) Expressed mouse Pemphigus Vulgaris antigen (PVA) by Baculovirus Expression System. 2) Generated monoclonal antibodies against human thyrotropin receptor (TSHR) and mouse PVA. 3) Researched on developing an animal model for autoimmune thyroid disease & Pemphigus Vulgaris in order to aid understanding the pathophysiology of these diseases and to develop specific immunotherapies. 4) Supervised post-doctoral fellows.

1992-1997 Postdoctoral Research fellow. Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston. 1) Evaluated B and T cell responses to thyrotropin receptor in human Graves' disease. 2) Generated and characterized a monoclonal antibody against the human PVA. 3) Contributed to discovery of cross reactivity of Yersinia Enterocolitica and thyroid thyrotropin receptor. 4) Promoted understanding molecular mechanism of human thyroid autoimmune disorders. 5) Expressed thyroid peroxidase (TPO) and Ku protein by Baculovirus expression system (TPO is being used in a commercial assay by General Biometrics Inc. in California). 6) Researched structure-function relationship of the human Pemphigus Vulgaris antigen protein. 7) Supervised a research associate, a graduate student, and a junior post-doctoral fellow.

1989-1991 Visiting Scientist. Tissue Typing Laboratory, Department of Surgery, University of California at Los Angeles. 1) Analyzed in HLA serologic epitopes. 2) Researched mechanisms of graft rejection.

1985-1989 Ph.D. Student. Department of Immunology, College of Medicine, Kyushu University, Fukuoka, Japan. 1) Researched drug-induced tolerance to allografs in mice. 2) Conducted treatment of autoimmune MRL/lpr mice with cholera toxin. 3) Studied on effect of lysimachia hemsleyana maxim in immune response.

1983-1985 Research Fellow. Institute of Epidemiology/Microbiology, Chinese Academy of Medicine. 1) Researched roles of macrophage in experimental leptospiral infection. 2) Studied intestinal microbiota and local immunity.

Awards

1994 The James W. McLaughlin Awards for Excellent Postdoctoral Fellow, University of Texas Medical Branch, TX.

1987-1988 Sun's Medical Research Awards, Ministry of Public Health, P. R. China.

Scholarships and Grants

1995-1999 NIH Grants DK47417, collaborated with P.I. Dr. Prabhakar.

1992-1994 The James W. McLaughlin Postdoctoral Fellowship, DHSS#5P30HD2784-02, University of Texas Medical Branch, Galveston, TX.

1986-1988 Japanese Government Research Fellowship for a Foreigner in Immunology, Kyushu University, Japan.

1985 WHO Research Fellowship in Immunology, Kyushu University, Japan.

Education

1989 Ph.D. in Microbiology/Immunology. Kyushu University, Fukuoka, Japan.

1983 M.S. in Microbiology/Immunology. Peking Union Medical College, Beijing, P. R. China.

1981 M.D., College of Medicine, Xian Jiaotong University, Xian, P.R.China.

Publications

J-L Fan and C. Yao. The role of macrophages in experimental leptospiral infection. Chinese J. Microbiol. Immunol. 4(1):17-21, 1984.

H. Mayumi, K. Himeno, K. Tanaka, N. Tokuda, J-L Fan and K. Nomoto . Drug-induced tolerance to allografs in mice. IX. Establishment of complete chimerism by allogeneic spleen cell transplantation from donors made tolerant to R-2 identical recipients. Transplant. 42:417- 422, 1986.

J-L Fan, H. Mayumi, N. Tokuda, K. Himeno and K. Nomoto. Treatment of autoimmune MRL/lpr mice with cholera toxin. Clin. Exp. Immunol. 70(1):94-l01, 1987.

J-L Fan, H. Mayumi, K. Himeno, N. Tokuda and K. Nomoto. Studies on lysimachia hemsleyana maxim. I. Effect of the drug on immune response. Journal Medicine & Pharmacology Society for WAKAN-YAKU (Chinese traditional medicine in Japanese). 4:88-93, 1987.

J-L Fan, K. Himeno and K. Nomoto. Studies on lysimachia hemsleyana maxim. II. Effect of the drug on auto immune MRL/lpr mice. Journal Medicine & Pharmacology Society for WAKAN-YAKU. 4:149-154, 1987.

H. Mayumi, K. Himeno, N. Tokuda, J-L Fan and K. Nomotoombination disparate at both 11-2 antigens by use of spleen cells from donors preimmuni. Drug-induced tolerance to allografis in mice. X. Augmentation of split tolerance in a murine c zed with recipient antigens. Immunobiol. 174(3):274-291. 1987.

J-L Fan, H. Mayumi, N. Tokuda, K. Himeno and K. Nomoto. Drug-induced tolerance to allografts in mice. XI. Tolerance induction using F I (donor X recipient) spleen cells as a tolerogen. Transplant. Proc. 19:3513-3519, 1987.

H. Mayumi, K. Himeno, K. Tanaka, J-L Fan and K. Nomoto. Drug-induced tolerance to allografis in mice. XII. The relationships between tolerance, chimerism and graft-versus host disease. Transplant. 44:286-290, 1987.

H. Mayumi, N. Tokuda, J-L Fan, K. Himeno and K. Nomoto.Drug-induced tolerance to allografis in mice. XIII. Tolerance to H-Y antigen. Transplant. Proc. 19:2975-2977, 1987.

N. Tokuda, H. Mayumi, K. Himeno, H. Gondo, J-L Fan and K. Nomoto. Drug-induced in vitro tolerance to allogeneic antigen. II. Further analysis of in vitro tolerized spleen cells in a fully allogeneic murine combination.Transplant. 45:464-470, 1988.

J-L Fan, K. Himeno, A. Hussain and K. Nomoto. Combined treatment of autoimmune MRLIlpr mice with cholera toxin plus irradiation. Biotherapy 1:97-102, 1989.

A. Barbetti, M. S. Park, P.I. Terasaki, B. Clark and J-L Fan. HLA serologic epitopes. In: Terasaki PI. ED. Clinical Transplants 1989. Los Angeles, 1990 by UCLA Tissue Typing Laboratory, Page 477-498.

G. Luo, J-L Fan, G. S. Seetharamaiah, R. K. Desai, J. S. Dallas, N.M. Wagle, R. Doan, D. W. Niesel, G. R. KIimpel and B. S. Prabhakar. Immunization of mice with Yersinia enterocolotoca leads to the induction of antithyrotropin receptor antibodies. J. Immunol. 151 (2):922-928, 1993.

J-L Fan, G.S. Seetharamaiah, R.K. Desai, J. S. Dallas, N.M. Wagle and B. S. Prabhakar. Analysis of autoantibody reactivity in patients with Gravest disease using recombinant extracellular domain of the human thyrotropin receptor and synthetic peptides. Autoimmunity, 15:285-291, 1993.

J-L Fan, R. K. Desai, G. S. Seetharamaiah, J. S. Dallas, N. M. Wagle and B. S. Prabhakar. Heterogeneity in cellular and antibody responses against thyrotropin receptor in patients with Graves' disease detected using synthetic peptides. J. Autoimmunity, 6:799-808, 1993.

R. K. Desai, J. S. Dallas, M. K. Gupta, G. S. Seetharamaiah, J-L Fan, K. Tahara, L. D. Kohn and B. S. Prabhakar. Dual mechanism of perturbation of thyrotropin-mediated activation of thyroid cells by antibodies to the thyrotropin eptor(TSHR) and TSHR¬derived peptides. J. Clin. Endocrinol. Metab., 77(3): 658-663, 1993.

J-L Fan, R. K. Desai, J. S. Dallas, G. S. Seetharamaiah and B. S. Prabhakar. High frequency of B cells capable of producing anti-thyrotropin receptor antibodies in patients with Graves' disease. Clin. Immunol. Immunopathol., 71(1): 69-74, 1994.

N. M. Wagle, J. S. Dallas, G. S. Seetharamaiah, J-L Fan, R. K. Desai, 0. Memar, S. Rajararnan and B. S. Prabhakar. Induction of hyperthyroxinemia in Balbic but not in other strains of mice. Autoimmunity, 18:103-112, 1994.

O. M. Memar, S. Rajaramari, R. Thotakura, S. K. Tyring, J-L Fan, G. S. Seetharamaiah, A. Lopez, R. E. Jordon and B. S. Prabhakar. Recombinant desmoglein 3 has the necessary epitopes to adsorb and induce blister-causing antibodies. J. Invest. Dermatol., 106:261-268, 1996.

J-L Fan, S. A. Patibaudla, K. Kimuna, T. N. Rao, R. K. Desai, G. S. Seetharamaiah, A. Kurosky and B. S. Prabhakar. Purification and characterization of a recombinant human thyroid peroxidase expressed in insect cell. J. Autoimmunity, 9:529-536, 1996.

S.A. Patibandla, N. M. Wagle, G. S. Seetharamaiah, J-L Fan, J. S. Dallas and B. S. Prabhakar. Experimental autoimmunity to thyrotropin receptor. Exp. Clin. Encrinol Diabetes 104, suppl 3, page 28-32, 1996.

0. Memar, B. Christensen, S. Rajararnan, R. Goldblum, S. K. Tying, M. M. Brysk, D. J. McCormick, H. Ei Zaim, J-L Fan, and B. S. Prabhakar. Induction of blister-causing antibodies by a recombinant full-length, but not the extracellular domain of the pemphigus vulgaris antigen (desinoglein 3). J. Immunol., 157:3171-3177, 1996.

G. S. Seetharamaiah, J-L Fan, S. A. Patibandla, and B. S. Prabbakar. Influence of adjuvants on the antibody response to a self and foreign antigen. Autoimmunity, 24:205-215, 1996.

S. A. Patibandla, J-L Fan, B. S. Prabhakar, G. S. Seetharamaiah. Cpmparison of immune responses to extracellular domains of mouse and human trhyrotropin receptor. J. Autoimmunity, 13(2):205-213, 1999.

S. Kaithamana, J-L Fan, Y. Osuga, S-G Liang, and B. S. Prabhakar. Induction of experimental autoimmune Graves’ disease in BALB/c mice. J. of Immunol. 163:5157-5164, 1999.

J-L Fan, 0. Memar, D. J. McCormick, and B. S. Prabhakar. BALB/c Mice produce blister causing antibodies upon immunization with a recombinant human desmoglein 3. J. Immunol. 163:6228-6235, 1999.

J-L Fan, J.W.Pertson, and B.S.Prabhakar. Adjuvant effects of Cholera toxin B subunit on immune response to recombinant thyrotropin receptor in mice. J.of Autoimmunity,14:43-52, 2000.

L.Curtis, J-L Fan, B-S Lee, D.Cai, I. Morozova, P.Scheff, V. Persky, C. Einoder, and S. Diblee. Pigeon allergens in indoor environments: a preliminary study. Allergy, 57:627-631, 2002.

S. Kaithamana ,J-L Fan, O. Memar, K. Li, J. Uitto, G.S. Seetharamaah, and B. S. Prabhakar. Relevance of differential immunogenicity of human and mouse recombinant mouse desmoglein-3 for the induction of acantholytic autoantibodies in mice. Clin. Exp. Immunol. 132:16-23, 2003.

L. Stojiljkovic, J-L Fan et al, Nuclear myosin I and B-actin are required for transcription by RNA polymerase II. Submitted to Science, 2006.

J-L Fan and T. Diekwisch. Isolation and characterization of a mouse CP27 protein expressed in E. Coli. (Prepare) 2006 J-L Fan, X. Luan, A. Yamane, and T.G.H. Diekwisch. Recombinant CP27 protein and temporo-spatial localization in developing craniofacial tissues. (Prepare) 2006.

J-L Fan, T.G.H. Diekwisch. Purification and characterization of a recombinant mouse amelogenin expressed in insect cell. (In prepare) 2006.

Reviews

J-L Fan. Intestinal microbiota and local immunity. External Medicine - Digestive system Disease fascicle 2:75-77, 1985.

J-L Fan and K. Himeno. Treatment of autoimmune disease with cholera toxin. Exp. Med. 5: 86-88, 1987.

B. S. Prabhakar, J-L Fan, G. S. Seetharamaiah. Thyrotropin-receptor-mediated diseases: A paradigm for receptor autoimmunity. Immunology Today, 1 8(9):437-442, 1997.

Books

J-L Fan. Bacteriocin. In: Bingyang Liu, ED. Bacteria-Past Present Future. Beijing: Chinese Science and Technical Publishing Company, Inc.; 1990:193-210.

Abstracts

J-L Fan, H. Mayumi, N. Tokuda, K. Himeno and K. Nomoto. Tolerance induction using F 1 (donor X recipient) spleen cells as a tolerogen. 15th Japanese Association of Immunology Annual Meeting, Fukuoka, Japan, 1985.

J-L Fan, H. Mayumi, N. Tokuda, K. Himeno and K. Nomoto. Treatment of autoimmune MRL/lpr mice with cholera toxin. 16th Japanese Association of Immunology Annual Meeting, Kyoto, Japan, 1986.

J-L Fan, H. Mayumi, K. Himeno, N. Tokuda and K. Nomoto. Studies on lysimachia herpsleyana WAKAN-YAKU (Chinese traditional maxim. I. Effect of the drug on immune response. 9th Japanese Medical Pharmacology Society for medicine in Japanese), Tokyo, Japan, 1986.

J-L Fan, K. Himeno, A. Hussain and K. Nomoto. Combined treatment of autoimmune MRL/lpr mice with cholera toxin plus irradiation. 17th Japanese Association of Immunology Annual Meeting, Kanezawa, Japan, 1987.

J-L Fan, K. Himeno and K. Nomoto. Studies on lysimachia hemsleyana maxim. II. Effect of the drug on autoimmune MRL/lpr mice. 1st Chinese-Japanese Congress of Chinese traditional Medicine, Tokyo, Japan, 1987.

H. Mayumi, N. Tokuda, J-L Fan, K. Himeno and K. Nomoto. Drug-induced tolerance to allografts in mice. XIII. Tolerance to H-Y antigen. 17th Japanese Association of Immunology Annual Meeting, Kanezawa, Japan, 1987.

J-L Fan, R. K. Desai, G.S. Seetharamaiah, J. S. Dallas, N. M. Wagle and B. S. Prabhakar. Patients with Graves' disease show heterogeneity in cellular and antibody responses against thyrotropin receptor. American Association of Immunologists Annual Meeting, Denver, CO, 1993.3. Immunol. 150 (8) Abstract Part II:74A.

N. M. Wagle, J-L Fan, G. S. Scetharamaiah, J. S. Dallas, R. K. Desai and B. S. Prabahkar. Experimental autoimmune Graves' disease: evaluation of immune response to thyrotropin receptor in mice. American Association of Immunologists Annual Meeting, Denver, CO, 1993.3. Immunol. 150(8) Abstract Part II:160A.

G.S. Seetharamaiah, R. K. Desai, J-L Fan, J. S. Dallas and B. S. Prabhakar. High level expression of extracellular domain of thyrotropin receptor: The autoantigen in Graves' disease. FASEB Annual Meeting, Anaheim, CA, 1992. The FASEB Journal 6(4) Part I: A1025.

R. K. Desai, J-L Fan and J. S. Dallas. Functional properties of polyclonal antithyrotropin receptor (TSHR) and anti-TSHR peptides antibodies. The 74th Annual Meeting of the Endocrine Society Program & Abstracts p162, San Antonio, TX, 1992.

J. S. Dallas G. S. Seethararnaiah, J-L Fan, S. J. Cunningham,R. M. Goldblum and B.S. Prabhakar. Identifleation of an immunodominant region on the thyrotropin (TSH) receptor: The autoantigen in Graves' disease. The 74th Annual Meeting of the Endocrine Society Program & Abstracts p63, San Antonio, TX, 1992.

J-L Fan, S. Patibandla, A. Kurosky and B. S. Prabhakar. High level baculovirus expression and IAPLC purification of human thyroid peroxidase. J. Immunol. 152(6); 32l7(48), 1994.

N. M. Wagle, J-L Fan, G. S. Seetharamaiah, J. S. Dallas, R. K. Desai, B .S. Prabhakar. Qualitative difference in antibody responses to thyrotropin receptor in mice that are susceptible and resistant to the development of experimental autoimmune Graves disease. FASEB Annual Meeting, Anaheim, CA 1994; The FASEB Journal 8(5) Part II: 978(5662).

J-L Fan, G. S. Seetharamaiah. Enzymatic activity of human thyroid peroxidase produced in insect cells in the presence of hematin. 11th International Thyroid Congress, Sep.10-15, 1995; Toronto, Canada.

J-L Fan, J. W. Peterson, B. S. Prabhakar. Effect of cholera toxin-B subunit on the development of experimental autoimmunity to TSH receptor. 10th International Congress of Endocrinology, June l2-15, 1996; S.F., CA, USA.

B. S. Prabhakar, G.S. Seethararnaiah, J. S. Dallas, J-L Fan, S. A. Patibandla, K. shashi. The immune response to the TSR receptor. 79th Annual Meeting of The Endocrine Society, June 11-14, 1997, Minneapolis, MN.

J-L Fan, X. Luan, A. Yamane, and T.G.H. Diekwisch. CP27 recombinant protein and temporo-spatial localization in developing craniofacial tissues. 8th International Conference The Chemistry and Biology of Mineralized Tissues. October 17-22, 2004, Banff Centre, Alberta, Canada.