CURRICULUM VITA
Christopher Halkides, Ph.D.
ADDRESS:
Dept of Chemistry
University of North Carolina- Wilmington
601 South College Road
Wilmington, N.C. 28403-3297
(910) 962-7427
HALKIDESC@UNCWIL.EDU
EDUCATION
Brandeis University, Waltham, MA 02254 -- Postdoctoral Research Associate Advisor: Prof. Alfred G. Redfield
characterized p21 ras (an oncoprotein) with NMR, elecron spin-echo envelope modulation (ESEEM), and EPR; made selectively labeled samples of p21Mn(II)GDP and p21Mn(II)GMPPNP and characterized p21 by NMR
Skills & Methodolgy: 2D NMR, selective labeling of bacterially expressed proteins, HPLC of
nucleotides, enzyme immobilization, chemical synthesis, interpretation of ESEEM spectra,
PCR and cloning,
Ph.D., 1990 -- University of Wisconsin-Madison, Madison, WI 53706
Thesis Advisor: Prof. Perry A. Frey
Major: Biochemistry Minor: Chemistry
synthesized the labile compound sym-monothiopyrophosphate (MTP), compared its reactivity
to pyrophosphate for PPi-dependent phosphofructokinase, characterized the rate of
nonenzymatic hydrolysis of MTP
Skills & Methodology: Chemical synthesis, chemical and enzymatic kinetics, heteronuclear
NMR, inorganic analysis
A.B., 1983 -- Wabash College, Crawfordsville, IN 47933 -- summa cum laude
Major: Chemistry Minor: Economics
AWARDS
National Institutes of Health Postdoctoral Fellow (1990-1993)
National Science Foundation Predoctoral Fellow (1984-1987)
MacIntosh Fellow of Wabash College
PROFESSIONAL MEMBERSHIPS
Phi Beta Kappa
American Chemical Society
EXPERIENCE
Academic Planning and Policy Committee of Wabash College (1981-1982)
Tutor, Thornton Community College (1979-1981)
PUBLICATIONS
Kenneth J. Gruys, Christopher J. Halkides, & Perry A. Frey; "Synthesis and Properties of 2-Acetylthiamin Pyrophosphate: An Enzymatic Reaction Intermediate," Biochemistry 26,
(1987), 7575-7585.
John M. Konopka, Christopher J. Halkides, Janeen L. Vanhooke, David G. Gorenstein, &
Perry A. Frey, "Uridinediphosphogalactose 4-epimerase. Phosphorus-31 nuclear magnetic
resonance analysis of NAD+ and NADH bound at the active site," Biochemistry 28, (1989),
2645.
Christopher J. Halkides & Perry A. Frey; "The Mechanism of Hydrolysis of -Monothiopyrophosphate," J. Am. Chem. Soc. 113 (1991), 9843-9848.
Christopher J. Halkides, Eric S. Lightcap, & Perry A. Frey; "The Chemical and Enzymatic
Reactivity of -Monothiopyrophosphate," Heteroatom Chemistry 2 (1991), 171.
Christopher J. Halkides, Matthew Benning, & Perry A. Frey; "The Structure of Tetramethyl
-Monothiopyrophosphate," Heteroatom Chemistry 2 (1991), 655.
Eric S. Lightcap, Christopher J. Halkides, & Perry A. Frey; "Interactions of Metal Ions with
-Monothiopyrophosphate," Biochemistry 30 (1991), 10307-10313.
Christopher J. Halkides, Eric S. Lightcap, & Perry A. Frey; "The Substrate Reactivity of -Monothiopyrophosphate with Pyrophosphate-Dependent Phosphofructokinase: Evidence for a
Dissociative Transition State in Enzymatic Phosphoryl Group Transfer," Biochemistry 30
(1991), 10313-10322.
Russell G. Larsen, Christopher J. Halkides, Alfred G. Redfield, & David J. Singel; "Electron
Spin Echo Envelope Modulation Spectroscopy of Mn2+GDP complexes of N-ras p21 with
Selective 15N Labeling," J. Am. Chem. Soc. 114 (1992), 9608-9611.
Russell G. Larsen, Christopher J. Halkides, & David J. Singel, "A Geometric Representation
of Nuclear Modulation Effects: The Effects of High Electron Spin Multiplicity on the ESEEM
Spectra of Mn(II) complexes of N-ras p21," J. Phys. Chem. 98 (1993), 6704-6721.
Anne-Frances Miller, Christopher J. Halkides, and Alfred G. Redfield, "Details of the
Conformational Switch of p21ras," Biochemistry 32 (1993), 7367-7376.
Christopher J. Halkides, John Tobin, & Perry A. Frey, "Observation of an Unusually Fast a-keto Deuteriation in Acetylthiamin Pyrophosphate and an Acetyl Thiazolium salt," J. Am.
Chem. Soc. 115 (1993), 3332.
Christopher J. Halkides, Christian T. Farrar, Russell G. Larsen, Alfred G. Redfield, & David
J. Singel, "Characterization of the Active Site of p21 ras by Electron Spin-Echo Envelope
Modulation Spectroscopy with Selective Labeling: Comparisons between GDP- and GTP-forms," Biochemistry 33 (1994), 4019-4035.
Christopher J. Halkides, Christian T. Farrar, & David J. Singel, "The Effects of
Cryoprotectants on the Activity and Structure of p21: Implications for Electron Spin-Echo
Envelope Modulation Spectroscopy," submitted for publication.
Christopher J. Halkides & Alfred G. Redfield, "The Effect of 17O on the Relaxation of an
Amide Proton Within a Hydrogen Bond," J. Biomol. NMR 5 (1995), 362-366.
Brendan F. Bellew, Christopher J. Halkides, Gary J. Gerfen, Robert G. Griffin, and David J.
Singel, "High Frequency Electron Paramagnetic Resonance Measurement of the Hydration in
Ternary Complexes of Mn(II), Guanine Nucleotides and ras p21," submitted for publication.
Christopher J. Halkides, Brendan F. Bellew, Gary J. Gerfen, Percy H. Carter, Bernice Ruo,
David A. Evans, Robert G. Griffin, and David J. Singel, "High Frequency (139.5 GHz)
Electron Paramagnetic Resonance Spectroscopy of the GTP Form of p21 ras with Selective
17O-labeling of Threonine, submitted for publication.
Christopher J. Halkides, Yong Qian Wu, and Christopher J. Murray, "A Low Barrier
Hydrogen Bond in Subtilisin Carlsberg: H-1 NMR Studies," submitted for publication.
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