CURRICULUM VITA

Deepak Shukla

EDUCATION:

Ph.D. candidate (1990-present) Microbiology and Immunology, University of Illinois, Chicago, IL
M.S. (1987-1989) Biochemistry, Banaras Hindu University, INDIA
B.S. (1983-1987) Chemistry (Major), Physics and Mathematics, Banaras Hindu University, INDIA

RESEARCH EXPERIENCE:

Graduate Student (08/90 - present), Department of Microbiology and Immunology, University of Illinois, Chicago, IL
Thesis Advisor: Dr. Philip Matsumura

Thesis research explores the structural-functional aspects of response regulator protein CheY in Escherichia coli chemotaxis.
*Developed a novel immunochemical assay to study protein-protein interactions in vitro.
* Demonstrated in vitro, the binding of response regulator protein CheY to histidine kinase CheA and, to switch protein FliM.
* Identified the surface of CheY involved in CheA interaction using engineered CheY mutants defective in CheA binding.
* Genetically determined the FliM binding face of CheY.
* Over-expressed and purified 16 proteins.
* Sequenced 25 different cheY clones using chain-termination method.

SKILLS:

DNA cloning, purification (HPLC), restriction analysis, deletion, site-specific and random mutagenesis, PCR, inverse-PCR, DNA sequencing, cell and tissue culture, protein over-expression, antibody and protein purification by size exclusion, ion-exchange, affinity and immunoaffinity chromatography, SDS-PAGE, spectrophotometry, protein-protein interaction assays including western blot, immunoprecipitation, fluorescence quenching, fluorescence polarization, and ELISA.

TEACHING EXPERIENCE:

Teaching Assistant (8/90-7/92)
Laboratory Instructor, Microbiology, College of Dentistry, University of Illinois, Chicago, IL
Responsibilities included lecturing, demonstration and lab supervision.

AWARDS AND HONORS:

Outstanding Research Presentation Award, 11th Annual Molecular Biology Retreat, University of Illinois, August 7-8, 1994, Lake Geneva, Wisconsin
A.S.M. Travel Grant Award, 94th General Meeting of the American Society for Microbiology, May 23-27, 1994, LasVegas, Nevada
Student Travel Award, College of Medicine, University of Illinois, Bacterial Locomotion and Signal Transduction Meeting, January 12-16, 1995, Austin, Texas
Qualified for graduate and post-graduate teaching in India through a nationwide competitive exam (NET), 1990, University Grants Commission, India

ABSTRACT PRESENTATIONS:

D. Shukla and P. Matsumura. Binding Interactions of CheA and CheY : a Surface on CheY that Binds to CheA is Determined by Enzyme-Linked Immunosorbent Assay. 94th General Meeting of American Society for Microbiology, May 23-27, 1994 LasVegas, Nevada

D. Shukla and P. Matsumura. Mutations in cheY Leading to Defective CheA Binding are Localized on a Surface of CheY. 11th Annual Molecular Biology Retreat, University of Illinois, Chicago, August 7-8, 1994, Lake Geneva, Wisconsin

D. Shukla and P. Matsumura. CheY: Kinase Binding Interface Overlaps with the Switch Binding Interface. 3rd Bacterial Locomotion and Signal Transduction Meeting, January 12-16, 1995 Austin, Texas


D. Shukla and P. Matsumura. FliM Binding Face of CheY as Determined by Genetic and Biochemical Assays. Gordon Conference on Sensory Transduction in Microorganisms, January 21-26, 1996 Ventura, California

SEMINARS/ORAL PRESENTATIONS:

"CheY-CheA Interactions" University of Illinois Prokaryotic Molecular Biology Meeting, November 1993, Department of Biology, University of Illinois, Chicago

"CheY-CheA Interactions and a Surface on CheY that Binds to CheA", Graduate Research Seminar Series, October 1994, Department of Microbiology and Immunology, University of Illinois, Chicago

"Understanding CheY: CheA and FliM Binding Surfaces Overlap", Graduate Research Seminar Series, July 1995, Department of Microbiology and Immunology, University of Illinois, Chicago

PUBLICATIONS:

D. Shukla and P. Matsumura." Mutations Leading to Altered CheA Binding Cluster on a Face of CheY" (1995) The Journal of Biological Chemistry, Vol.270, N0. 41, pp24414-24419.

D. Shukla and P. Matsumura." FliM Binding Face of CheY as Determined by Genetic and Biochemical assays" (in preparation).

PROFESSIONAL REFERENCES:

Available upon request.

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