YE ZHU, M.D.

Postdoctoral Research Associate

Ye Zhu

RESEARCH INTEREST:

How does E.coli transctriptional activator FlhD regulate cell division through induction of PH regulator CadA? FlhD, transcribed from flhD flagellar master operon, not only is required to express other flagellar genes, but also reduces cell division in response to acid stress.By using transposon mutagenesis,which transcriptionally regulated by FlhD, we found fused gene 97% identity with CadA. Cad operon, induced by low PH, consists of CadA (encoding lysine decarboxylase), CadB(encoding lysine/cadaverin antiporter), Cad C(encoding regultor of CadA&CadB).

My approach is:
1. to see whether FlhD directly binds CadA, using gel-shift assay, Dnase footprint,
and in vitro transcription assay.
2. If no direct interaction between FlhD and CadA, use transposon mutagenesis assay to figure out whether any other factors involve in regulation of cell division by induction of CadA.

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