CURRICULUM VITAE

 

Andrew (Andy) David Mesecar

 

Center for Pharmaceutical Biotechnology

 and Department of Medicinal Chemistry & Pharmacognosy

University of Illinois at Chicago

900 South Ashland Avenue (M/C 870)

Chicago, IL 60607-7173

 

Telephone: 312-996-1877

Fax 312-413-9303

e-mail: mesecar@uic.edu

 

EDUCATION

 

University of California                 Post-Doctoral Fellow    1995-1999

Berkeley, CA.                                      

University of Notre Dame             Ph.D. Biochemistry        1995

Notre Dame, IN.

Purdue University                         B.S. Chemistry               1988

West Lafayette, IN.

 

EXPERIENCE

University of Illinois, Chicago (August 2004-present) Associate Professor, Department of Medicinal Chemistry and Pharmacognosy and the Center for Pharmaceutical Biotechnology. 

 

University of Illinois, Chicago (1999-2004) Assistant Professor, Department of Medicinal Chemistry and Pharmacognosy and the Center for Pharmaceutical Biotechnology. 

 

University of California, Berkeley (1995 - 1999) Postdoctoral Research Fellow under Professor Daniel E. Koshland Jr. -Department of Molecular and Cell Biology.   Research focused on mapping the structural changes that occur along the reaction trajectory of an enzyme-catalyzed reaction by using a combination of methods including cryogenic x-ray crystallography, site-directed mutagenesis, enzyme kinetics and chemical modification.

 

University of Notre Dame (1988 -1994)  Graduate Research Scientist under Professor Thomas Nowak -Department of Chemistry and Biochemistry.  Dissertation Title: "Kinetic Responses and Conformational Changes Required for Yeast Pyruvate Kinase Activation and Catalysis".  Research focused on constructing a kinetic and thermodynamic model for the allosteric regulation of yeast pyruvate kinase and identifying amino acid residues involved in catalysis and regulation.

 

Purdue University (1987 -1988)  Undergraduate Research Assistant under Professor Robert F. Brush -Department of Psychobiology and Endocrinology.  Research focused on correlating rat adrenal gland size, weight and in-vitro corticosteroid output with rat behavioral responses, i.e. low or high tolerances to stress and pain.

 

Purdue University (1986 - 1988) Undergraduate Research Assistant under Professor David Gorenstein -Department of Chemistry.  Research focused on the isolation and purification of the lac repressor head-piece for NMR structure determination.

 

FELLOWSHIPS, SCHOLARSHIPS AND AWARDS

*     American Association of Colleges of Pharmacy New Investigator Award                                                                                        1999 - 2000

*     U.S. Department of Energy: Post-Doctoral Fellowship                      1995 - 1998

*     Center for Advanced Materials, Lawrence Berkeley National Laboratory

*     Reilly Graduate Fellowship: University of Notre Dame                     1993 - 1994

*     U.S. Department of Education: Graduate Fellowship

*     GAANNP Scholarship Program                                                                                                                                       1989 - 1992

*     U. S. Air Force ROTC Four-Year Scholarship (used only one year)    1984 - 1988

 

MEMBERSHIPS AND PROFESSIONAL AFFILIATIONS

*     American Chemical Society-Division of Biological Chemistry

*     American Association for the Advancement of Science

*     Protein Society

*     American Crystallographic Association

*     Alpha Chi Sigma-National Chemistry Fraternity

 

PROFESSIONAL COURSES TAUGHT

*     American Crystallographic Association Summer School in X-ray Crystallography (2003-2004).                                                        

 

PROFESSIONAL COURSES ATTENDED

*     Cold Spring Harbor Course in Macromolecular Crystallography (1999).

 

SCIENTIFIC ADVISORY BOARDS

*     Shamrock Structures, LLC. Woodridge, IL. (2003-present)

 

PROFESSIONAL NOMINATIONS

*     Institute for Defense Analyses.  Nomination to participate in the Defense Science Group (DSSG) program for 2004 to 2005. (2003)

 

PUBLICATIONS

  • Mesecar, A. D., Stoddard, B.L. and Koshland, D. E. (1997) Orbital Steering in the Catalytic Power of Enzymes:  Small Structural Changes with Large Catalytic Consequences. Science, Vol. 277, pp. 202-206.
  • Mesecar, A. D. and Nowak, T. (1997) Metal-Ion-Mediated Allosteric Triggering of Yeast Pyruvate Kinase. 1. A Multidimensional Kinetic Linked-Function Analysis. Biochemistry, Vol. 36, Num. 22, pp. 6792-6802.
  • Mesecar, A. D. and Nowak, T. (1997) Metal-Ion-Mediated Allosteric Triggering of Yeast Pyruvate Kinase. 2. A Multidimensional Thermodynamic Linked-Function Analysis. Biochemistry, Vol. 36, Num. 22, pp. 6803-6813.
  • Jurica, M. S., Mesecar, A. D., Heath, P. J., Shi, W., Nowak,T. and Stoddard, B. L. (1998) The Allosteric Activation of Pyruvate Kinase by Fructose-1, 6-bisphosphate.  Structure, Vol. 6, No 2 pp. 195-210.
  • Stoddard, B.L, Cohen, B., Brubaker, M., Mesecar, A.D., and Koshland, D.E. Jr. (1998) Millisecond Laue structures of an enzyme-product complex using photocaged substrate analogs. Nature Structural Biology,Vol 5, No 10 pp.891-897.
  • Bollenbach, T., Mesecar, A.D. and Nowak, T. (1999) The role of lysine-240 in the mechanism of yeast pyruvate kinase.  Biochemistry, Vol 28, No 28, pp 9137-45.
  • Peters-Wendisch, P., Rombel, I., Mesecar, A.D.,  Thorgeirsson, T., Shin, Y.-K. and Kustu, S. (1999) MgATP binding and hydrolysis determinants of NtrC, a bacterial enhancer-binding protein. Journal of Bacteriology, Vol 181, No 15 pp.4628-38.
  • Freiberg, G., Mesecar, A.D., Huang, H. and Liebman, S.W. (2000) Characterization of novel rad6/ubc2 ubiquitin-conjugating enzyme mutants in yeast.  Current Genetics, Vol. 37 No 4, pp. 221-33.
  • Mesecar, A..D. and Koshland, D.E. (2000). A four location model to explain the stereospecificity of proteins. Nature, 403, 614-615.
  • Mesecar, A..D. and Koshland, D.E. (2000). Sites of binding and orientation in a four location model for protein stereospecificity. IUBMB-Life, Vol 49, No 5, pp. 457-466.Bond, C.J., Jurica, M.S., Mesecar, A.D. and Stoddard, B.L. (2000). The determinants of allosteric activation of yeast pyruvate kinase and the identification of novel effectors using computational screening. Biochemistry, 39, 15333-15343.

·       Velichutina, I.V., Hong, J.Y., Mesecar, A.D., Chernoff, Y.O., and Liebman, S.W. (2001).  Genetic interaction between yeast Saccharmoyces cerevisiae release factors and the decoding region of the 18S rRNA. J. Mol. Biol., 305(4):715-727.

·       Su, B.-N., Misico, R., Park, E. J., Santarsiero, B. D., Mesecar, A. D., Fong, H. H. S., Pezzuto, J. M., and Kinghorn, A. D. (2002) "Isolation and characterization of bioactive principles of the leaves and stems of Physalis philadelphica (Tomatillos)", Tetrahedron 58(17), 3453-3466.

·       Cui, J., Marankan, F., Crich, D. Mesecar, A.D. and Johnson, M.E. (2002).  An oxyanion-hole selective serine protease inhibitor in complex with trypsin.  Bioorganic and Medicinal Chemistry, 10(1), 41-46.

·       Su, B.-N., Chang, L. C., Park, E. J., Cuendet, M., Santarsiero, B. D., Mesecar, A. D., Mehta, R. G., Fong, H. H. S., Pezzuto, J. M., and Kinghorn, A. D. (2002)  "Bioactive Constituents of the Seeds of Brucea javanica," Planta Medica, 68, 730-733.

·       Chen, S-N, Li, W., Fabricant, D.S., Santarsiero, B.D., Mesecar, A.D., Fitzloff, J.F., Fong, H.H.S. and Farnsworth, N.R. (2002) "Isolation, structure elucidation and absolute configuration of 23-epi-26-deoxyactein from Cimicifuga racemosa and clarification of nomenclature associated with 27-deoxyactein. J. Nat. Prod. 65(4), 601-605.

·       Li S-H., Zhang, H-J., Qiu, S-X., Niu, X-M., Santarsiero, B.D., Mesecar, A. D. Fong, H. H. S., Farnsworth, N.R. and Sun, H-D. "Vitexlactam A, a novel labdane diterpene lactam from the fruits of Vitex angnus-castus." (2002) Tetrahedron Letters, 43(29), 5131-5134.

·       Jang, D.S., Park, E.J., Hawthorne, M.E., Vigo, J.S., Graham, J.G., Cabieses, F., Santarsiero, B.D., Mesecar, A.D., Fong, H.S., Mehta, R. G., Pezzuto, J.M., and Kinghorn, A.D. (2002). Constituents of Musa x paradisiaca Cultivar with the potential to induce the phase II enzyme, quinone reductase.  J. Agricultural and Food Chem. Oct 23;50(22):6330-4.

·       Su, B.-N., Park, E.J., Mbwambo, Z.H., Santarsiero, B., Mesecar, A.D., Fong, H.S., Pezzuto, J. M. and Kinghorn, A.D. (2002) "New chemical constituents of Euphorbia quinquecostata and absolute configuration assignment by a convenient Mosher ester procedure carried out in NMR tubes. J. Nat. Prod. Sep;65(9):1278-82.

·        Zhang, X., Gan, L., Pan, H., Guo, S., He, X., Olson, S. T., Mesecar, A. D., Adam, S., and Unterman, T. G. (2002). Phosphorylation of serine 256 suppresses transactivation by FKHR (FOXO1) by multiple mechanisms: Direct and indirect effects on nuclear/cytoplasmic shuttling and DNA binding.  J. Biological Chemistry. Nov 22;277(47):45276-84.

·       Park, S., Hayes, B.L., Marankan, F., Mulhearn, D., Wanna, L., Mesecar, A., Santarsiero, B., Johnson, M. E., Venton, D. L. (2003) Regioselective covalent modification of hemoglobin in search of antisickling agents. J. Medicinal Chemistry. 46(6):936-53

·       Su, B-N., Park, E. J., Nikolic, D., Santarsiero, B. D., Mesecar, A. D., Vigo, J. S., Graham, J. G., Cabieses, F., van Breemen, R. B., Fong, H. H. S., Farnsworth, N. R., Pezzuto, J. M., and Kinghorn, D.  (2003). Activity-guided isolation of novel norwithanolides from Deprea subtriflora with potential cancer chemopreventive activity.  J. Organic Chemistry. 21;68(6):2350-61.

·       Rao, K.V., Santarsiero, B. D., Mesecar, A.D., Schinazi, R.F., Tekwani, B.L. and Hamann M.T. (2003). New manzamine alkaloids with activity against infectious and tropical parasitic diseases from an Indonesian Sponge. Journal of Natural Products, 66(6), 823-828.

·       Jang, D. S., Park, E. J., Hawthorne, M. E., Vigo, J. S., Graham, J. G. , Cabieses, F., Santarsiero, B. D., Mesecar, A. D., Fong, H. H. S., Mehta, R. G., Pezzuto, J. M., and Kinghorn, A. D. (2003) Potential cancer chemopreventive constituents of the seeds of Dipteryx odorata (tonka bean).  Journal of Natural Products, 66(5), 583-587.

·       Yao, J., Li, Y., Chang, M., Wu, H., Goodman, J., Liu, X., Liu, H., Mesecar, A. D., van Breemen, R., Yager, J. D., and Bolton, J (2003). Catechol estrogen 4-hydroxyequilenin is a substrate and an inhibitor of catechol-o-methyltransferase.  Chem. Res. Toxicol., 16(5), 668-675.

·       Zhang, H-J., Tan, G. T., Santarsiero, B. D., Mesecar, A. D., Hung, M. V., Cuong, N. M., Soejarto, D. D., Pezzuto, J. M., and Fong, H. H. S. (2003). New sesquiterpenes from Litsea verticillata. Journal of Natural Products. 66(5), 609-615.

·       Burdette, J. E., Liu, J., Chen, S-n., Fabricant, D. S., Piersen, C. E., Barker, E. L., Pezzuto, J. M., Mesecar, A. D., van Breemen, R., Farnsworth, N. R., and Bolton, J. L. (2003) "Black cohosh acts a mixed competitive ligand and partial agonist of the serotonin receptor". J. Agriculture and Food. Chem. Sep 10;51(19):5661-5670.

·        Su, B-N., Kang, Y-H., Pinos, R. E., Santarsiero, B. D., Mesecar, A. D., Soejarto, D. D., Fong, H. H. S., Pezzuto, J. M. and Kinghorn, D. (2003). Isolation and absolute stereochemistry of coussaric acid, a new bioactive triterpenoid from the stems of Coussarea brevicaulis.  Phytochemistry, Sep;64(1):293-302.

·       Graham, J. G., Zhang, H., Pendland, S., Santarsiero, B., Mesecar, A.D., Gabieses, F., Farnsworth, N. R., (2004)  "Antimycobacterial napthopyrones from Senna obliqua" J. Nat. Prod., Feb;67(2):225-7.

·        Yan Li, Jiaqin Yao, Minsun Chang, Dejan Nikolic, Linning Yu, James D. Yarger, Andrew D. Mesecar, Richard B. van Breemen, and Judy L. Bolton. (2004) "Equine catechol estrogen 4-hydroxyequilenin is a more potent inhibitor of the variant form of catechol-o-methyltransferase. Chem. Res. Toxicol., Apr 19;17(4):512-520.

·       Bang Yeon Hwang, Bao-Ning Su, Heebyung Chai, Qiuwen Mi, Leonardus B.S. Kardono, Johar J. Afriastini, Soedarsono Riswan, Bernard D. Santarsiero, Andrew D. Mesecar, Craig R. Fairchild, Robert Wild, William C. Rose, Gregory D. Vite, Norman R. Farnsworth, Geoffrey A. Cordell, John M. Pezzuto, Steven M. Swanson and A. Douglas Kinghorn. (2004). "Silvestrol and episilvestrol, novel rocaglate derivatives from Aglaia silvestris with potential anticancer activity. J. Organic Chemistry. May 14;69(10):3350-3358.

·       Nguyen Q.C., Nguyen VH, Santarsiero, B.D., Mesecar, A.D., Nguyen M. C., Soejarto, D.D.,  Pezzuto, J.M., Fong, H.H., and Tan, G.T.. (2004) "New 3-O-acyl betulinic acids from Strychnos vanprukii" Journal of Natural Products, Jun;67(6):994-998.

·       Sochanchingwung Rumthao, Oukseub Lee, Qi Sheng, David Crich, Andrew D. Mesecar and Michael E. Johnson. (2004) "Design, synthesis and evaluation of oxyanion-hole selective inhibitor substituents for the S1 subsite of Factor Xa". Bioorganic & Medicinal Chemistry Letters. 14, 5165-5170.

·       Chien, N. Q., Tan, G. T., Hung, N. V., Santarsiero, B. D., Mesecar, A. D., Cuong, N. M., Soejarto, D. D., Pezzuto, J., M.,and Fong, H. H. S. (2004)  "Natural Anti-HIV Agents.  New 3-O-Acyl betulinic acids from Strychnos wallichiana Steud. ex DC. (Loganiaceae)," Submitted.

·       Dombrauckas, J.D., Santarsiero, B.D., Mesecar, A.D. (2005) “Structural Basis Tumor-Pyuvate Kinase M2 Allosteric Regulation and Catalysis.   Biochemistry (submitted & under minor revision).

 

UNIVERSITY AND PROFESSIONAL ACTIVITIES AND SERVICE (UIC)

 

COLLEGE COMMITTEES

 

*     Image and Public Relations Committee (2003 to present).  College of Pharmacy. Chairperson.  Appointed by Dean.

*     Student Faculty Relations Committee (2001 to 2003). College of Pharmacy.  Elected Position.

*     Student Discipline Committee (2003 to 2004). College of Pharmacy. Elected Position..

*     Search Committee (2003) for Head, Department of Medicinal Chemistry and Pharmacognosy (Ad Hoc).

*     American Council on Pharmaceutical Education (ACPE) Self-Study Committee (April to November, 2001) .Ad Hoc Member-Appointed by the Dean.  Worked on gathering and assembling the faculty information for the College Self-Study Report. Service Dates.

 

DEPARTMENTAL AND CENTER COMMITTEES AND ACTIVITIES

 

Faculty Advisory Committee (2003 to Present). Department of Medicinal Chemistry and Pharmacognosy. Elected Position.

 

Program Chair, Structural Biology Track, Medicinal Chemistry Graduate Program (2001-Present).  We have restructured our graduate program to include structural biology and enzymology components in many of the graduate courses  to emphasize the importance of structural biology and enzymology in drug design and molecular recognition processes.  My primary roles are to guide faculty who are course coordinators in the implementation of relevent structural biology and enzymology components into their curriculum, and to organize student rotations through the various faculty members of the track. 

 

Search Committee (2003 to Present)  for Medicinal Chemistry and Pharmacognosy faculty positions.  (Ad Hoc)

 

Department Webmaster (2001-Present).  I continue to be in charge of construction, reconstruction, and maintenance of the Department of Medicinal Chemistry and Pharmacognosy website.

 

Center for Pharmaceutical Biotechnology-Recruiting Committee Chair (August 2000 to 2001).  Appointed by Dr. Michael Johnson to organize a committee to establish a recruiting strategy for the Center and for the Department of Medicinal Chemistry and Pharmacognosy. Service Dates.

 

Search Committee (2001) for NMR Spectroscopist position, Department of Medicinal Chemistry and Pharmacognosy.

 

Comprehensive Exam Committee (June, 2000 and 2002)-Written Exam. Medicinal Chemistry Graduate Program. Member.  Was responsible or creation and grading of a portion of the year 2000 Preliminary Written Exam.

 

Medicinal Chemistry Graduate Program Revision Committee (2000-2001).  Member.  Worked on a plan to revise the Medicinal Chemistry Graduate program.  Program was implemented in Fall of 2002.

 

Faculty Sponsor, Undergraduate Student Research Outreach Program, Center for Pharmaceutical Biotechnology (2001).  I initiated an outreach program for undergraduate students attending other colleges in the Chicago area in order to give them the opportunity to conduct research projects that they may not be able to pursue at their respective colleges.  The program is designed to serve two functions: First, it will give students in the biological and chemical sciences the opportunity to conduct high quality research in a University setting that they may not otherwise be able to conduct at their respective college.  Second, it allows us to identify and recruit high quality and underrepresented students for our graduate research programs. That year one student worked in my laboratory and subsequently joined the Pharmacognosy graduate program.

 

UNIVERSITY COMMITTEES AND SERVICE

 

University of Illinois, Board of Governors for CARS (2002 to Present).   I am currently serving as an alternate member to the Board of Governors for Consortium for Advances Radiation Sources (CARS) at the Advanced Photon Source, Argonne National Laboratory.  The University of Illinois at Chicago has become an institutional member of this Consortium.

 

Faculty Advisory Committee, UIC Center for Structural Biology (2000 to Present). My role is to provide advice to the Director on how to fulfill the missions of the Center in the areas of Teaching Initiatives, Research Initiatives, and Facilities Development.

 

Faculty Advisory and User Committee-Macromolecular Crystallography Facility (2000 to Present).  Committee member for Research Resources Center to oversee the operations and usage of x-ray diffraction instrumentation.

 

Faculty Advisory and User Committee-Small Molecule X-ray Diffraction Facility (2002 to Present).  Committee member for Research Resources Center to oversee the operations and usage of x-ray diffraction instrumentation.

 

Search Committee (2001). for a scientific coordinator between UIC and the Consortium for Advances Radiation Sources (CARS) at the Advanced Photon Source, Argonne National Laboratory.

 

PROFESSIONAL SCIENTIFIC COMMITTEES

 

Executive Committee-Chairman (2003-Present) for the Instrument Development Team (IDHT) for "MaNDi" a Macromolecular Neutron Diffractometer at the Spallation Neutron Source (SNS).  The MaNDi IDT has been allocated a sector at the SNS at Oakridge National Laboratory to build a world renowned, best-in-class neutron diffractometer for macromolecular neutron structures.  The IDT is currently trying to raise funds in the amount of $10-15 million for construction of MaNDi. 

 

Steering Committee-(2002-2003) A United States national committee of prominant scientists was formed to develop a plan to build a state-of-the-art Neutron Diffraction Facility at the Spallation Neutron Source (SNS) at Oakridge National Laboratory.

 

PROFESSIONAL GRANT REVIEW PANELS

 

NIH Study Sections (2001 to present)

-National Center for Research Resources (NCRR) Study Section-2003 to Present

-Chemical Pathology Study Section (Ad hoc, 2001, 2002, 2003).

-National Center for Research Resources (NCRR) Study Section-2001 (Ad Hoc-Site Review).

 

Journal Reviewer (1999-Present)-Ad Hoc.  Nature Structure and Molecular Biology, Proceedings from the National Academy of Sciences, Biochemistry, Journal of Biological Chemistry, Applied and Environmental Microbiology, Chemical Research in Toxicology, Combinatorial Chemistry and High Throughput Screening, Protein Science.

 

Proposal Review Panel (2001-Present) Advanced Photon Source, Argonne National Laboratory. I review proposals for requests for synchrotron beamtime.

 

Proposal Reviewer (2003,2004)-Research Corporation (Ad hoc), GenomeCandada (Ad Hoc)

 

 

PROFESSIONAL SCIENTIFIC CONFERENCES ORGANIZED

 

23rd Midwest Enzyme Chemistry Conference (October, 2003)-Local Chairman & UIC site host.  October 4th, 2003.  This meeting will be hosted in the college of Pharmacy.  There are approximately 200 registered participants and over 100 posters and presentations. Forthcoming.

A Workshop for MaNDi: a Macromolecular Neutron Diffractometer at the Spallation Neutron Source (October, 2003).-Workshop Organizer.  This workshop will be held from October 2nd to 3rd, 2003 at the Intense Pulsed Neutron Source at Argonne National Laboratory.  Forthcoming.

Session Chair, "Time Resolved Diffraction in Chemistry & Biology Symposium" (July, 2003).  American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31. 

Session Chair,  "Macromolecular Motions and Dynamic Processes." (May, 2002).  American Crystallographic Association, Annual Meeting, San Antonio, Texas.  May 25th - 30th.

 

 

STUDENTS AND POST-DOCTORAL RESEARCH FELLOWS MENTORED AT UIC

 

1.    Qi Sheng (2000 to 2003).  Graduated from my lab with a Masters of Science in Pharmacognosy. She finished her thesis work in December 2003 and graduated formally in May 2003.  Thesis Title: “Accelerating Structure-based Drug Design of Factor Xa Inhibitors Via Engineered Bovine”.

2.    Sasi Kiran Chilukuri (2000 to present)  Ph.D. student in the Pharmacognosy Graduate Program.

3.    Jill Dombrauckas (2001 to present) Ph.D. student in the Medicinal Chemistry Graduate Program.

4.    Barbara Calamini (2001 to present) Ph.D. student in the Medicinal Chemistry Graduate Program.

5.    Sonia Larsen (2001 to present) Ph.D. student in the Pharmacognosy Graduate Program

6.    Patrick Miller (2002 to present) Ph.D. student in the Pharmacognosy Graduate Program

7.    Kiira Ratia (2003 to present) Ph.D. student in the Pharmacognosy Graduate Program

8.    Gary Klein (2004 to present) Ph.D. student in the Medicinal Chemistry Graduate Program

9.    Renda Hawwa (2004 to present) Ph.D. student in the Medicinal Chemistry Graduate Program

10.  Valerie Sershon (2004 to present) Ph.D. student in the Medicinal Chemistry Graduate Program.

11.  Yang Tian (2004 to present) Ph.D. student in the Pharmacognosy Graduate Program

12.  Magdalini Vamvouka, Ph.D. (January, 2000 to August, 2003) Post-doctoral research associate.

13.  Aimee Eggler, Ph.D. (April, 2003 to present) Post-doctoral Research Associate.

14.  Melissa May Co-Advised with Dr. Mike Johnson

15.  Huidong Yu Co-Advised with Dr. Mike Johnson

 

INVITED SEMINARS AND SYMPOSIA

 

1.    Andrew Mesecar, Kiira Ratia, Bernard Santarsiero, Kai Xi, Dalia Jukneliene, Brian Harcourt, Paul Rota, Susan Baker, Arun Ghosh (2004). "Crystallographic and Kinetic Studies of Novel SARS-CoV 3CLpro Protease Inhibitors that Inhibit SARS-CoV and MHV-A59 Replication". The 7th International Symposium on Positive-Strand RNA Viruses.  San Francisco, CA.  May 27-June 1. Plenary Program..

2.    Mesecar, A. D. (2004) "Molecular Movies of Enzyme Catalysis: Implications for Dynamic, Structure-Based Drug Design”.  Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN.  May 3rd.

3.    Mesecar, A. D. (2004) "Crystallographic Studies of Enzyme Reaction Coordinates: Molecular Odysseys in Four Dimensions". Department of Chemistry, University of Toledo. April 5th.

4.    Mesecar, A. D. (2004) "A new organophosphorous hydrolase from Deinococcus radiodurans".  NATO Army Armaments Group.  Project Group 31 on "Non-Corrosive, Biotechnology-Based Decontaminants fro CBW Agents. Eighteenth Meeting held at the McGowan Institute for Regenerative Medicine, Pittsburgh, PA.  March 30 -31.

5.    Mesecar, A. D. (2003) "Settling Controversies in Enzyme Catalysis via Macromolecular Neutron Diffraction". Los Alamos Neutron Science Center, Los Alamos National Laboratory, Los Alamos, NM.  Sixth User Group Meeting.  October 19 - 21.

6.    Mesecar, A. D. (2003) "Enzyme Catalyzed Reactions in Four Dimensions". The University of Iowa, Iowa City, IA. Department of Biochemistry.  September 25th.

7.    Mesecar, A.D. (2003) "Dynamic motions of enzymes revealed by static and time-resolved x-ray structures".  The University of Minnesota, Minneapolis, MN. Department of Biochemistry, Molecular Biology and Biophysics (BMMB).  October 8th.

8.    Mesecar, A.D., Barry Stoddard, Bernie Santarsiero, Sonia Larsen, Kiira Ratia, Magdalini Vamvouka, Zhong Ren, Reinhard Pahl and Vukica Srajer. (2003) "Static and Time-Resolved Studies of Enzyme Reaction Coordinates: Molecular Odysseys in Four Dimensions".  Time Resolved Diffraction in Chemistry & Biology Symposium.  American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.

9.    Mesecar, A.D. (2003) "Prospecting the biosphere for novel enzyme functions"  Fred Hutchinson Cancer Research Center.  Basic Sciences Division. Seattle, WA. Monday, May 19th, 2003.

10.Mesecar, A.D. (2002) "Dynamic motions of enzymes during catalysis revealed by time-resolved x-ray structures"  University of Illinois at Chicago, Department of Biochemistry. Chicago, Illinois. Thursday, November 21st.

11.Mesecar, A.D. (2002) "Making molecular movies of enzyme reaction coordinates.  This isn't Hollywood. XXII Midwest Enzyme Chemistry Conference.  Chicago, Illinois. Saturday, September 28th.

12.Mesecar, A.D. (2002) "A new paradigm for rational drug design: Thinking out of the static, active site box".  MediChem, Inc. Woodridge, Illinois. Thursday, March 7th.

13.Mesecar, A.D. (2001) Molecular Movies of Enzyme Action:  This isn’t Hollywood.  Purdue University. West Lafayette, Indiana. Structural Biology Seminar Series. Department of Biology.Wednesday, November 14th.

14.Mesecar, A.D. (2001) Molecular Movies and Enzyme Reaction Coordinates.  The 11th Users Meeting of the Advanced Photon Source, Argonne National Laboratory. Argonne, Illinois. Tuesday, October 9th. 

15.Mesecar, A.D. (2001) Molecular Movies of Enzyme Action:  This isn’t Hollywood.  Northern Illinois University. DeKalb, Illinois. Monday, September 10th. Graduate Colloquium Seminar. 

16.Mesecar, A.D. (2001)  "Making Molecular Movies of Enzyme Catalysis: This isn't Hollywood"  BioCARS Workshop on Time-Resolved Crystallography. Monday, March 12th. Argonne National laboratory, Argonne, Illinois.

17.Mesecar, A.D. (2001)  "The Experiment: Caging Strategies for Triggering Enzyme Reaction in the Crystalline State "  BioCARS Workshop on Time-Resolved Crystallography. Tuesday, March 13th.  Argonne National laboratory, Argonne, Illinois..

18.Mesecar, A.D. (2001)  "Rational Drug Design: Past, Present and Future"  Forty-Ninth Annual Honors Convocation.  Thursday, February 22nd. College of Pharmacy, University of Illinois at Chicago. Chicago, Illinois. Honors Address-Invited Speaker.

19.Mesecar, A.D. (2001) "A New Paradigm for Rational Drug Design".  Vahlteich Award Recipient Series. Friday, March 2nd. University of Illinois at Chicago. Chicago, Illinois.

20.Mesecar, A.D. (2000)  "Molecular Movies of Enzymes in Action." Friday, November 17th.  Medical College of Wisconsin.

21.Mesecar, A.D. (2000).  "Creating a Molecular Movie of an Enzyme Catalyzed Reaction."  Finch University of Health Sciences/The Chicago Medical School. Thursday, February 24th.

22.Mesecar, A.D. (1999).  "Protein Conformational Changes: How small is Big Enough?  University of Wisconsin, Madison." Friday, October 29th.

23.Mesecar, A.D. (1999).  "Protein Conformational Changes: How small is Big Enough?"  Illinois Institute of Technology, Chicago, Illinois. Monday, November 15th.

24.Mesecar, A.D. (1999).  "Protein Conformational Changes: How small is Big Enough?"  University of Notre Dame.  June 10th and 11th.

25.Mesecar, A.D. (1996)"Revealing the Regulatory Role of the Divalent Metal of the Pyruvate Kinase Reaction by a Complete Thermodynamic Analysis of the Ligand Binding Parameters" (1995).  University Federal do Rio de Janeiro, Brazil.

26.Mesecar, A. D. and Nowak, T. (1993) "Non-concerted Allosteric Behavior of Yeast Pyruvate Kinase." XIIIth Midwest Enzyme Chemistry Conference (1993).

 

CONFERENCE AND SYMPOSIA-ABSTRACTS

 

1.    Li, Yan; Yao, Jiaqin; Chang, Minsun; Yu, Linning; Yager, James D.; Mesecar, Andrew D.; van Breemen, Richard B.; Bolton, Judy.  "Equine estrogen metabolite 4-hydroxyequilenin (4-OHEN) is a more potent inhibitor of the variant form of catechol O-methyltransferase (COMT)".    Abstracts of Papers, 226th ACS National Meeting, New York, NY, United States, September 7-11, 2003  (2003),     TOXI-071.

2.    Chang, Minsun; Calamini, Barbara; Nikolic, Dejan; Ratia, Kiira; van Breemen, Richard B.; Mesecar, Andrew D.; Bolton, Judy L.  "Differential modification of the ligand binding domains of human estrogen receptors alpha and beta by the equine estrogen metabolite, 4-hydroxyequilenin."    Abstracts of Papers, 226th ACS National Meeting, New York, NY, United States, September 7-11, 2003  (2003),     TOXI-060.

3.    Sonia Larsen, Kiira Ratia, Bernard Santarsiero and Andrew Mesecar. (2003) "Monochromatic and Polychromatic Laue Studies of a Novel Phosphotriesterase that Degrades Soman Gas." Center for Pharmaceutical Biotechnology, Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

4.    Barbara Calamini*, Bernie Santarsiero*, Kiira Ratia*, Michael Malkowski§, John Pezzuto#, and Andrew Mesecar*.  (2003) Structural Basis for Potent Inhibition of Cox-1 and Cox-2 by Resveratrol-A Natural Product in Wine.*Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, §Hauptman-Woodward Medical Research Institute, and #Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

5.     Jill Dombrauckas, Bernard Sanstersiero, and  Andrew D. Mesecar. (2003) "X-ray structure determination of tumor-specific pyruvate kinase type-M2 from humans". Department of Medicinal Chemistry, University of Illinois at Chicago; 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

6.    Kiira Ratia  and Andrew Mesecar. (2003) Directed Evolution of a Novel Phosphotriesterase Reveals Dynamic Contributions to Catalysis. Center for Pharmaceutical Biotechnology and the Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

7.    Sasi K. Chilukuri, Bernard D. Santarsiero, and Andrew D. Mesecar. (2003) Structure of Pyruvate Kinase with a Novel Allosteric Activator Identified via Computational Docking. Center for Pharmaceutical Biotechnology, Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

8.    Magdalini Vamvouka and Andrew Mesecar (2003) High Resolution Structures of THT-Dioxygenase Mutants: Insight into Extradiol Catalysis., The Center for Pharmaceutical Biotechnology and the Dept. of Medicinal Chemistry and Pharmacognosy, The Univ. of Illinois at Chicago. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

9.    Bernard Santarsiero, Kiira Ratia, and Andrew D. Mesecar. (2003) A Comparison of Bromide Ion "Quick Soak" and Se-Met Derivatives for Structure Determination of a Novel Hydrolase. The Center for Pharmaceutical Biotechnology and the Dept. of Medicinal Chemistry and Pharmacognosy, The Univ. of Illinois at Chicago. 23rd Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, October 4th. Poster.

10.Sonia Larsen, Kiira Ratia, Bernard Santarsiero and Andrew Mesecar. (2003) Monochromatic and Polychromatic Laue Studies of a Novel Phosphotriesterase that Degrades Soman Gas. Center for Pharmaceutical Biotechnology, Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Invited oral presentation.

11.Barbara Calamini*, Bernie Santarsiero*, Kiira Ratia*, Michael Malkowski§, John Pezzuto#, and Andrew Mesecar*.  (2003) Structural Basis for Potent Inhibition of Cox-1 and Cox-2 by Resveratrol-A Natural Product in Wine.*Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, §Hauptman-Woodward Medical Research Institute, and #Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

12.Jill Dombrauckas1, Dagny L. Ulrich2, Bernard Sanstersiero1, Pat Loria2, Andrew D. Mesecar1. (2003) DeNovo X-ray Structure Determination of the Bacterial Quorum-Sensing Phoshorelay Protein Lux U. 1Department of Medicinal Chemistry, University of Illinois at Chicago; 2Department of Chemistry, Yale University. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

13.Kiira Ratia  and Andrew Mesecar. (2003) Directed Evolution of a Novel Phosphotriesterase Reveals Dynamic Contributions to Catalysis. Center for Pharmaceutical Biotechnology and the Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

14.Sasi K. Chilukuri, Bernard D. Santarsiero, and Andrew D. Mesecar. (2003) Structure of Pyruvate Kinase with a Novel Allosteric Activator Identified via Computational Docking. Center for Pharmaceutical Biotechnology, Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

15.Magdalini Vamvouka and Andrew Mesecar (2003) High Resolution Structures of THT-Dioxygenase Mutants: Insight into Extradiol Catalysis., The Center for Pharmaceutical Biotechnology and the Dept. of Medicinal Chemistry and Pharmacognosy, The Univ. of Illinois at Chicago. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

16.Bernard Santarsiero, Kiira Ratia, and Andrew D. Mesecar. (2003) A Comparison of Bromide Ion "Quick Soak" and Se-Met Derivatives for Structure Determination of a Novel Hydrolase. The Center for Pharmaceutical Biotechnology and the Dept. of Medicinal Chemistry and Pharmacognosy, The Univ. of Illinois at Chicago. American Crystallographic Society Meeting. Covington, Kentucky, July 26 - 31.  Poster.

17.Su, B-N, Kang, Y-H, Pinos, R. E., Santarsiero, B. D., Mesecar, A. D., Soejarto, D. D., Fong, H.H.S., Pezzuto, J. M., and Kinghorn, A. D. (2003). Isolation and absolute stereochemistry of coussaric acid, a new bioactive triterpenoid from the stems of Coussarea brevicaulis. 44th Annual Meeting of the American Society of Pharmacognosy "The Changing Face of Natural Products Chemistry". Chapel Hill, NC. July 12-16.

18.Calamini, B. and Mesecar, A.D. (2002). A structural basis for chemopreventive activity of resveratrol-a natural product of wine. XXII Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, September 28th. Poster.

19.Sheng, Q. Johnson, M. and Mesecar, A. D. (2002). Accelerating structure-based drug design of factor Xa inhibitors via engineered bovine trypsin. XXII Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, September 28th. Poster.

20.Vamvouka, M. and Mesecar, A. D. (2002). A proton shuttle mechanism for extradiol dioxygenases revealed by high resolution crystal structures and kinetic characterization of site-directed mutants. XXII Midwest Enzyme Chemistry Conference. Chicago, Illinois. Saturday, September 28th. Poster.

21.Grubjesic, S., Santersiero, B., Kosmeder II, J.W., Gigli, H., Moriarty, R.M., and Mesecar, A.D. Synthesis of caged catechol substrates for time resolved Laue x-ray studies of trihydroxytoluene dioxygenase. Abstracts of Papers, 224th National Meeting of the American Chemical Society, Boston, MA, August 18-22, 2002; MEDI 182.

22.Santarsiero, B.D., Mesecar, A.D., Su, B,-N., Fong H.H.S., Pezzuto, J.M., Kinghorn, A.D. (2002) "Using 109 molecules and a synchrotron to determine a crystal structure". American Crystallographic Association, Annual Meeting, San Antonio, Texas.  May 25th - 30th. Oral Paper Presentation.

23.Su, B. N., Park, E.J., Vigo, J.S., Graham, J.G., Cabieses, F., Santarsiero, B., Mesecar, A.D., Fong, H. H. S., Farnsworth, N.R., Pezzuto, J. M., Kinghorn, A.D. (2002) "Activity-guided isolation and cancer chemopreventive potential of unusual 18-norwithanolides with a new type of carbon skeleton from Solanum altissiumum." 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium. July 27th-31st. New Brunswick, New Jersey. Poster

24.Su, B.N. Park, E.J., Mbwambo, Z. H., Santarsiero, B., Mesecar, A.D., Fong, H. H. S., Pezzuto, J. M., Kinghorn, A.D. (2002) "Chemical constituents of Euphorbia quinquecostata and absolute configuration assignment by a Mosher ester procedure conveniently carried out in a NMR tube. 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium. July 27th-31st. New Brunswick, New Jersey. Poster

25.Jang, D. S., Park, E. J., Hawthorn, M.E., Vigo, J. S., Graham, J.G., Cabieses, F., Santarsiero, B.D., Mesecar, A.D., Fong, H.H.S., Mehta, R.G., Pezzuto, J.M. and Kinghorn, A.D. (2002) "Constituents of Musa Sapientum (Banana) with the potential to induce the phase II enzyme, quinoine reductase. 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium. July 27th-31st. New Brunswick, New Jersey. Poster.

26.Ratia, K., Falzarim, K., Santarsiero, B. D. and Mesecar, A.D.  (2001) Kinetic and thermal characterization of pyruvate kinase mutants associated with hemolytic anemia. XXI Midwest Enzyme Chemistry Conference. Poster.

27.Gunter, H. and Mesecar, A.D. (2001) Evidnece for a CO2 Binding pocket in isocitrate dehydrogenase. XXI Midwest Enzyme Chemistry Conference. Poster.

28.Santarsiero, B.D. and Mesecar, A.D. (2001) New insights into extradiol ring-cleaving mechanisms as revealed from the by the x-ray structures of trihydroxytoluene dioxygenase. The native structure at 1.20Å and the 4-Nitrocatechol complex at 1.20Å. XXI Midwest Enzyme Chemistry Conference. Poster.

29.Simonida Grubjesic, Bernard Santersiero,  Jerry Kosmeder, Zhong Ren, Reinhard Pahl, and Andrew Mesecar. (2001) Trihydroxytoluene dioxygenase as a model system for time-resolved laue x-ray diffraction studies. XXI Midwest Enzyme Chemistry Conference. Poster.

30.Su, B-N, Chang, L.C., Park, E.J., Cuendet, M., Santarsiero, B.D., Mesecar, A.D., Mehta, R.G., Fong, H.H.S., Pezzuto, J.M. and Kinghorn, A.D. (2001) Bioactive constituents of the seeds of Brucea javanica. Amer. Chem. Soc. Natl. Meeting. Chicago. MEDI: Abst. #0134. Poster.

31.Santarsiero, B., Ren, Z. Pahl, R. and Mesecar, A.D. (2001)  Monochromatic and Laue Structural Studies of Trihydroxytoluene Dioxygenase.  BioCARS Workshop on Time-Resolved Crystallography.  Argonne National laboratory.  Poster.

32.Mesecar, A.D. (2001)  A Molecular Movie of the isocitrate dehydrogenase reaction."  BioCARS Workshop on Time-Resolved Crystallography.  Argonne National laboratory.  Poster.

33.Kloss, P. and Mesecar, A.D. (2000). Over-expression and purification of an FAD utilizing monooxygenase involved in bioremediation of dinitrotoluene (DNT). XXth Midwest Enzyme Chemistry Conference. Poster.

34.Binutu, O. and Mesecar, A.D. (2000). Crystallization and preliminary x-ray characterization of a ring cleaving dioxygenase involved in bioremediation of dinitrotoluene. XXth Midwest Enzyme Chemistry Conference. Poster.

35.Mesecar, A.D. and Koshland, D.E. (2000). A New Model for Protein Stereospecificity. 14th Symposium of the Protein Society, August 5 - 9, 2000, in San Diego, California.  Abstract #00441, Protein Science.

36.Mesecar, A. D. and Daniel E. Koshland Jr.  (1999). X-ray structural evidence for metal-ion dependent enantiomeric specificity of isocitrate dehydrogenase: "Four-Point Location" in enzymatic stereospecificity.  XIXth Midwest Enzyme Chemistry Conference.

37.Barry L. Stoddard, Jill Bolduc, and Dave Dyer (The Fred Hutchinson Cancer Research Center) and  Andrew Mesecar, Bruce Cohen, Mike Brubaker, and Daniel E. Koshland, Jr. (University of California, Berkeley) "Time-resolved Crystallographic Studies of Isocitrate Dehydrogenase: Using Intermediate Trapping, Photolytic Triggering, and Synchrotron Radiation". The Sixth International Conference on Biophysics and Synchrotron Radiation, August 4-8, 1998, Advanced Photon Source,  Argonne National Laboratory, USA.

38.Mesecar, A. D., Stoddard, B. L. and Daniel E. Koshland, Jr.  (1997) Small Conformational Changes of an Enzyme Under Catalytic Turnover:  The Isocitrate Dehydrogenase Catalyzed Reaction..  17th IUBMB/ASBMB Conference-San Francisco.  FASEB Journal, v. 11, n.9, A2672.

39.Mesecar, A. D. Larry, J. P.  and Nowak, T. (1995). The Function of Active Site Residues in Pyruvate Kinase. ASBMB/DBC-ACS Joint Meeting-San Francisco. FASEB Journal, v.9, n.6,: A1286. Poster.

40.Mesecar, A. D. and Nowak, T. (1994) The Role of Lysine-269 in the Pyruvate Kinase Reaction. XIVth Midwest Enzyme Chemistry Conference. Poster.

41.Mesecar, A.D. and Nowak, T. (1993) Non-Concerted Allosteric Interactions of phosphoenolpyruvate,  fructose 1,6-diphosphate and  Mn2+ with Pyruvate Kinase from Saccharomyces cerevisiae. ASBMB/DBC-ACS Joint Meeting-San Diego. FASEB Journal, v.7, n.7: A1102.

42.Mesecar, A. D. and Nowak, T. (1992) Allosteric Interactions of phosphoenolpyruvate and fructose 1,6-diphosphate with Mn2+ activated pyruvate kinase from Saccharomyces cerevisiae. XIIth Midwest Enzyme Chemistry Conference. Poster

43.Mesecar, A.D. and Nowak, T.   Kinetic and Genetic Studies of Yeast Pyruvate Kinase. (1992) ASBMB/Biophysical Society Joint Meeting-Houston, Biophysical Journal, v.61, n.2 Part 2: A331.

44.Mesecar, A. D. and Nowak, T. (1991). Lysine-269 in the Pyruvate Kinase Reaction:  pH Studies and Site-Directed Mutagenesis. XIth Midwest Enzyme Chemistry Conference. Poster.

45.Mesecar, A. D. and Nowak, T. (1990)  pH Studies on Yeast Pyruvate Kinase:  Mn2+ Activation and the Substrate Phosphoenolpyruvate. Xth Midwest Enzyme Chemistry Conference. Poster.

46.Mesecar, A. D. and Nowak, T. (1989) pH Effects on Mn2+ Activation of Yeast Pyruvate Kinase IXth Midwest Enzyme Chemistry Conference. Poster