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QINGBO LI
qkli@uic.edu
312-413-9301
Assistant Professor
Ph.D., Iowa State
University, 1995
We are interested in the role and the pathway of
anaerobic respiration by M. tuberculosis for intracellular growth
and persistence. Even though M. tuberculosis is classified as an
obligate aerobe, its successful survival in macrophages and granuloma suggests that it may carry out anaerobic
respiration. Indeed, the genome of M. tuberculosis contains two
anaerobic nitrate reductase gene clusters, in
addition to a large number of genes for fatty acid metabolism and lipid biosynthesis.
The ability for M. tuberculosis to carry out anaerobic respiration
is probably a critical molecular basis for long term persistence. Currently
there is a paucity of information about the protein expression of M.
tuberculosis in infected macrophages. Recent advances of proteomics,
particularly in quantitation capability, have
provided great promise of benefits to biology community. Given that cells
are dynamic with metabolisms that can react to an ever-changing environment, our true understanding of a proteome will
require information of both dynamic and homeostatic mechanisms, plus an
understanding of the governing intra- and extra-cellular components,
including host immune response. Using proteomics approach, we expect to
define the proteins critical for M. tuberculosis persistence in infected macrophages and
granuloma tissues, and the implication of these
proteins for drug discovery.
L. Li, Q. Li,
L. Rohlin, U. Kim, K. Salmon, T. Rejtar, R. P. Gunsalus, B. L.
Karger, and J. G. Ferry. 2007. Quantitative proteomic and microarray analysis of the archaeon
Methanosarcina acetivorans grown
with acetate versus methanol. J
Proteome Res 6(2):759-71.
D. J.
Lessner, L. Li, Q. Li, T. Rejtar, V. P. Andreev, M. Reichlen, K.
Hill, J. J. Moran, B. L. Karger, and J. G. Ferry. 2006. An unconventional
pathway for the reduction of CO2 to methane in CO-grown Methanosarcina acetivorans
revealed by proteomics. Proc Natl Acad Sci U S A 103(47):17921-6.
V.P. Andreev, L. Li, T. Rejtar, Q.
Li, J. G. Ferry, and B. L. Karger. 2006. New algorithm for 15N/14N
quantitation with LC-ESI-MS using an LTQ-FT mass
spectrometer. J Proteome Res 5(8):2039-45.
Q. Li, L. Li,
T. Rejtar, D. J. Lessner, B. L. Karger, and J. G.
Ferry. 2006. Electron transport in the pathway of acetate conversion to
methane in the marine archaeon Methanosarcina acetivorans.
J Bacteriol 188(2):702-10.
Q. Li, L. Li, T. Rejtar, B. L. Karger, and
J. G. Ferry. 2005. Proteome of Methanosarcina acetivorans.
Part II, comparison of protein abundance in acetate- and methanol-grown
cells. J Proteome Res 4(1):129-35.
Q. Li, L. Li, T. Rejtar, B. L. Karger, and
J. G. Ferry. 2005. Proteome of Methanosarcina acetivorans.
Part I, an expanded view of the biology of the cell. J Proteome Res 4(1):112-28.
C. T. Culiat, M. L. Klebig,
Z. Liu, H. Monroe, B. Stanford, J. Desai, S. Tandan,
L. Hughes, M. K. Kerley, D. A. Carpenter, D. K.
Johnson, E. M. Rinchik, and Q. Li. 2005. Identification of mutations from
phenotype-driven ENU mutagenesis in mouse Chromosome 7. Mamm
Genome 16(8):555-66.
Q. Li, C. Deka, B. J. Glassner, K. Arnold,
X.-C. Li-Sucholeiki, A. Tomita-Mitchell, W. G. Thilly, and B. L. Karger. 2005. Design of an automated multicapillary
instrument with fraction collector for DNA mutation discovery by constant
denaturant capillary electrophoresis (CDCE). J Sep Science 28(12): 1375-89.
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