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Imunnoassay Examples

These are examples of immunoassays that could be developed and performed in the Core Biological Laboratory for persons associated with the Center for Reducing Risks in Vulnerable Populations (CRRVP).

This is a partial list of biological markers and signals that can be measured in the College of Nursing’s Core Biological Laboratory. If an investigator is interested in measuring other “markers” in biological fluids please contact Linda Graham, at 312-413-3695 or lgraham@uic.edu.

AD = Assay Designs, Ann Arbor, MI	DSL = Diagnostic Systems Laboratories Inc., Webster, TX
ALPCO = American Laboratory Products Company, Windham, NH	IBL= IB Lab, Hamburg, Germany
B-RL = Bio-Rad Labs, Hercules, CA	ICN = ICN Diagnostic Product, Irvine, CA
CD = Cortez Diagnostics, Calabasa, CA	PL = Peninsula Lab, Belmont, CA
DPC = Diagnostic Products Corporation, Los Angeles, CA	DZL = Diazyme Laboratories, San Diego, CA

Signal Marker	Sample Source	Sample Size	Type of Assay	Company
Reproductive Hormones
Male
Testosterone	Serum, Plasma	0.1 ml	RIA, EIA	DSL, ICN
Dihydro-Testosterone	Serum, Plasma	0.1 ml	RIA	DSL
Free Testosterone	Serum, Plasma	0.1 ml	RIA, EIA	DSL
Androstenedione	Serum, Plasma	0.1 ml	RIA, EIA	DSL, DPC, ICN
Androstanediol Glucuronide	Serum, Plasma	0.1 ml	RIA, EIA	DSL
DHEA, DHEA-S	Serum, Plasma, Saliva	0.025 - 0.2ml	RIA, EIA	DSL, DPC, ICN
Female
17ß Estradiol	Serum, Plasma	0.1ml, 0.05ml	RIA, EIA	DSL, DPC, ICN
Estrone	Serum, Plasma	0.05ml - 0.1ml	RIA, EIA	DSL, ICN
Free Estriol	Serum, Plasma	0.025ml - 0.05ml	EIA	ICN
Progesterone	Serum, Plasma	0.025ml - 0.05ml	RIA, EIA	DSL, ICN
17aHydroxyprogesterone	Serum, Plasma	0.05ml	RIA, EIA	DSL, ICN
Other
SHBG (sex hormone binding globulin)	Serum	0.05ml, 0.025ml	RIA, IRMA	DSL
FSH	Serum, Plasma	0.1ml	RIA, ELISA	DSL, DPC, ICN
LH	Serum, Plasma, Urine	0.025ml, 0.05ml, 0.2ml	IRMA, ELISA, EIA	DSL, DPC, ICN
Prolactin	Serum	0.025ml	IRMA, ELISA, EIA	DSL, DPC, ICN
Oxytocin	Serum, Plasma	0.1ml	EIA	AD
Hypothalmic/Pituitary/ Adrenal Axis (stress response)
ACTH	Plasma	0.1ml - 0.2ml	ELISA, IRMA, RIA	DSL, DPC, ICN
Cortisol	Serum, Saliva, Urine	0.025ml	RIA, EIA	DSL, DPC, ICN
Corticotropin Releasing Factor	Inquire	Inquire	RIA	PL
Also see catecholamines
Renin/Angiotensin/Aldosterone (RAS and fluid balance)
Renin	Serum, Plasma	0.2ml	IRMA	DSL
Aldosterone	Plasma, Serum, Urine	0.1ml	RIA, EIA	DSL, DPC, ICN
Arginine vasopression	Plasma	0.3ml	RIA	DSL
Bone/Mineral Density
Calcitonin	Serum, Plasma	0.1ml - 0.3ml	RIA, ELISA, IRMA	DSL
PTH (parathyroid hormone)	Serum, Plasma	0.05ml - 0.2ml	RIA, ELISA, IRMA	DSL
Growth Factors
IGF–I, II (insulin-like growth factor)	Serum, Plasma	0.02ml - 0.05ml	ELISA, IRMA	DSL
Free IGF-I, II	Serum, Plasma	0.025ml - 0.1ml	ELISA, IRMA	DSL
Human Growth Hormone hGH	Serum	0.020ml - 0.1ml	ELISA, IRMA, RIA	DSL, DPC, ICN
hGH Binding Protein (human growth hormone binding protein)	Serum	0.1ml	ELISA	DSL
Erythropoietin	Serum, Plasma	0.1ml	RIA	DSL
Metabolism
Insulin	Serum, Plasma	0.1ml	RIA, ELISA	DSL, DPC, ICN
Human Leptin	Serum, Plasma	0.02ml - 0.1ml	IRMA, ELISA	DSL
Total T3	Serum, Plasma	0.05ml	RIA, EIA	DSL, ICN
Free T3	Serum	0.05ml	EIA, RIA	DSL, ICN
Total T4	Serum	0.05ml	RIA, EIA	DSL, ICN
Free T4	Serum	0.05ml	EIA, RIA	DSL, ICN
Thyroid stimulating hormone	Serum	0.1ml , 0.05ml	ELISA, IRMA, RIA	DSL, ICN
Thyroid Uptake	Serum	0.025ml	EIA, RIA	ICN
C-Peptide of insulin	Serum, Plasma, Urine	0.025ml, 0.1	EIA, RIA	ALPCO, DSL
Inflammation/Infection
CRP (C-Reactive Protein)	Serum	0.05ml	ELISA	DSL
IL (Interleukin) 1ß, a	Serum, Plasma	0.05ml	EIA	ALPCO
IL2, IL 2R	Serum, Plasma	0.05ml	EIA	ALPCO
IL 4, 5, 6, 8, 10, 13, 14, 15	Serum, Plasma	0.05ml	EIA	ALPCO
VEGF (vascular endothelial growth factor)	Serum, Plasma	0.05ml	EIA	ALPCO
Prostaglandins D2, E1, E2, F2a	Serum, Saliva, Urine, Blood	0.1ml	EIA	AD
Leukotrienes B4	Serum, Saliva, Urine	0.1ml	EIA	AD
Endocrine/Other
B-endorphin	Plasma, Tissue	3.0ml	RIA	ALPCO
Substance P	Plasma	2.0ml	RIA	ALPCO
Somatostatin	Plasma	1.0ml	RIA	ALPCO
Melatonin	Serum, Plasma, Saliva	0.2ml	EIA	ALPCO
Cholecystokinin	Plasma Tissue	1.0ml	RIA	ALPCO
Catecholamines
Norepinephrine	Plasma, Urine, Tissue	0.3ml	RIA	ALPCO
Epinephrine	Plasma, Urine, Tissue	0.3ml	RIA	ALPCO
Dopamine	Plasma, Urine, Tissue	0.6ml	RIA	ALPCO
Cardiac
CRP (C-reactive protein)	Serum	0.01ml	ELISA	DSL
Homocysteine	Serum, Plasma	Inquire	Microplate	DZT
Creatine phosphokinase	Serum, Plasma	0.025ml	ELISA	IBL, B-RL
Cardiac Troponin, Myoglobin and Creatine kinase	Serum, Plasma	0.1ml	Immunoassay	CD
Endothelin	Serum	0.05ml	EIA/Other	ALPCO, AD
Nitric Oxide	Inquire	Inquire	Microplate	AD
Tumor Necrosis Factor (TNF a)	Serum, Plasma, Saliva, Urine	Inquire	EIA	ALPCO
Iron Metabolism
Ferritin	Serum, Plasma	0.5ml	IRMA	DSL, DPC, ICN
Tumor Markers
AFP a-Fetoprotein	Serum	0.025ml	ELISA, IRMA	DSL, ICN
CA 125 Ovarian Cancer Antigen	Serum	0.1ml	ELISA	ICN
CA 15-3 Breast Cancer Antigen	Serum	0.2ml	ELISA	ICN
CA 19-9 Gastrointestinal Cancer Antigen	Serum	0.1ml	ELISA	ICN
CEA Carcinoembryonic Antigen	Serum	0.025ml	ELISA	DSL, ICN
PSA Prostate Specific Antigen Free/Total	Serum	0.02ml - 0.05ml	ELISA	DSL, ICN